Efficient multiplication of human metapneumovirus in Vero cells expressing the transmembrane serine protease TMPRSS2

被引：142

作者：

Shirogane, Yuta ^{[1
]}

Takeda, Makoto ^{[1
]}

Iwasaki, Masaharu ^{[1
]}

Ishiguro, Nobuhisa ^{[2
]}

Takeuchi, Hiroki ^{[1
]}

Nakatsu, Yuichiro ^{[1
]}

Tahara, Maino ^{[1
]}

Kikuta, Hideaki ^{[3
]}

Yanagi, Yusuke ^{[1
]}

机构：

[1] Kyushu Univ, Fac Med, Dept Virol, Fukuoka 8128582, Japan

[2] Hokkaido Univ, Grad Sch Med, Dept Pediat, Sapporo, Hokkaido 0608638, Japan

[3] Touei Hosp, Pediat Clin, Higashi Ku, Sapporo, Hokkaido 0070841, Japan

来源：

JOURNAL OF VIROLOGY | 2008年 / 82卷 / 17期

关键词：

D O I：

10.1128/JVI.00676-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human metapneumovirus (HMPV) is a major causative agent of severe bronchiolitis and pneumonia. Its fusion (F) protein must be cleaved by host proteases to cause membrane fusion, a critical step for virus infection. By generating Vero cells constitutively expressing the transmembrane serine protease TMPRSS2 and green fluorescent protein-expressing recombinant HMPV, we show that TMPRSS2, which is expressed in the human lung epithelium, cleaves the HMPV F protein efficiently and supports HMPV multiplication. The results indicate that TMPRSS2 is a possible candidate protease involved in the development of lower respiratory tract illness in HMPV-infected patients.

引用

页码：8942 / 8946

页数：5

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