Identification and Evaluation of Autoantibody to a Novel Tumor-Associated Antigen GNA11 as a Biomarker in Esophageal Squamous Cell Carcinoma

被引:3
|
作者
Wang, Huimin [1 ,2 ,3 ,4 ,5 ]
Yang, Xiaoang [1 ]
Sun, Guiying [3 ,4 ,5 ]
Yang, Qian [3 ,4 ,5 ]
Cui, Chi [1 ,3 ,4 ]
Wang, Xiao [1 ,3 ,4 ]
Ye, Hua [3 ,4 ,5 ]
Dai, Liping [1 ,3 ,4 ]
Shi, Jianxiang [1 ,3 ,4 ]
Zhang, Jianying [1 ,3 ,4 ]
Wang, Peng [1 ,3 ,4 ,5 ]
机构
[1] Zhengzhou Univ, Acad Med Sci, Henan Inst Med & Pharmaceut Sci, Zhengzhou, Peoples R China
[2] Zhengzhou Univ, Sch Basic Med Sci, Zhengzhou, Peoples R China
[3] Zhengzhou Univ, Henan Key Lab Tumor Epidemiol, Zhengzhou, Peoples R China
[4] Zhengzhou Univ, State Key Lab Esophageal Canc Prevent & Treatment, Zhengzhou, Peoples R China
[5] Zhengzhou Univ, Coll Publ Hlth, Zhengzhou, Peoples R China
来源
FRONTIERS IN ONCOLOGY | 2021年 / 11卷
关键词
GNA11; tumor-associated antigen; autoantibody; immunodiagnosis; esophageal squamous cell carcinoma; DIAGNOSTIC BIOMARKER; CANCER; EXPRESSION; REPORTERS;
D O I
10.3389/fonc.2021.661043
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The study aims to explore the diagnostic value of anti-GNA11 autoantibody in esophageal squamous cell carcinoma (ESCC) from multiple levels. Autoantibody against GNA11 with the highest diagnostic performance was screened out from the customized protein microarray. A total of 486 subjects including ESCC patients and matched normal controls were recruited in the verification and validation phases by using enzyme-linked immunosorbent assay (ELISA). Western blotting analysis was used to verify the ELISA results. Immunohistochemistry (IHC) was used to evaluate GNA11 expression in ESCC tissues and para-tumor tissues. In addition, a bioinformatics approach was adopted to investigate the mRNA expression of GNA11 in ESCC. Results indicated that the level of anti-GNA11 autoantibody in ESCC patients was significantly higher than that in the normal controls, and it can be used to distinguish ESCC patients from normal individuals in clinical subgroups (p < 0.05), as revealed by both ELISA and Western blotting. The receiver operating characteristic (ROC) curve analysis showed that anti-GNA11 autoantibody could distinguish ESCC patients from normal controls with an area under the ROC curve (AUC) of 0.653, sensitivity of 10.96%, and specificity of 98.63% in the verification cohort and with an AUC of 0.751, sensitivity of 38.24%, and specificity of 88.82% in the validation cohort. IHC manifested that the expression of GNA11 can differentiate ESCC tissues with para-tumor tissues (p < 0.05), but it cannot be used to differentiate different pathological grades and clinical stages (p > 0.05). The mRNA expression of GNA11 in ESCC patients and normal controls was different with a bioinformatics mining with The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) data in Gene Expression Profiling Interactive Analysis (GEPIA). In summary, anti-GNA11 autoantibody has the potential to be a new serological marker in the diagnosis of ESCC.
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页数:12
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