Discrimination of DNA duplexes with matched and mismatched tandem repeats by T4 endonuclease VII

被引：0
作者：
Surdi, GA
Yaar, R
Smith, CL
机构：
[1] Boston Univ, Ctr Adv Biotechnol, Dept Biomed Engn, Boston, MA 02215 USA
[2] Boston Univ, Ctr Adv Biotechnol, Dept Biol, Boston, MA 02215 USA
[3] Boston Univ, Ctr Adv Biotechnol, Dept Pharmacol & Expt Therapeut, Boston, MA 02215 USA
来源：
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1999年 / 14卷 / 5-6期
关键词：
D O I：
10.1016/S1050-3862(98)00022-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple in situ method for scoring short tandem DNA repeat length has been developed using T4 endonuclease VII. This method measures tandem repeated simple sequences embedded in unique sequences. Single-stranded loops are formed on duplexes containing mismatched (different) numbers of tandem repeats. No single stranded loops are formed on structures containing matched (identical) numbers of tandem repeats. The matched and mismatched loop structures were distinguished and differentially labeled by enzymatic treatment with T4 endonuclease VII. (C) 1999 Elsevier Science B.V. All rights reserved.
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页码：177 / 179
页数：3
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