A multiplex-PCR to identify hepatitis B virus -: genotypes A-F

被引：50
作者：
Kirschberg, O
Schüttler, C
Repp, R
Schaefer, S
机构：
[1] Univ Giessen, Inst Med Virol, Giessen, Germany
[2] Univ Erlangen Nurnberg, Klin Kinder & Jugendliche, Nurnberg, Germany
来源：
JOURNAL OF CLINICAL VIROLOGY | 2004年 / 29卷 / 01期
关键词：
multiplex-PCR; hepatitis B virus; genotypes A-F;
D O I：
10.1016/S1386-6532(03)00084-2
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Eight genotypes (A-H) of hepatitis B virus (HBV) are known with variations in nucleotide sequences greater than 8%. Several recent publications found that the clinical course and outcome of antiviral therapy depended on the genotype of the infecting HBV strain. Large epidemiological studies will require the availability of a system which is rapid, reliable and can be performed on a large number of samples. We have developed a multiplex-PCR assay which uses genotype-specific primer pairs for HBV genotypes A-F. These primer pairs specifically amplified HBV DNA of the respective genotype, either in single or in multiplex-PCR. Sensitivity of the assay was in the range of 10(4) genome equivalents. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码：39 / 43
页数：5
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