Cloning and characterization of 37 kDa laminin receptor precursor in pearl oyster, Pinctada fucata

被引:3
|
作者
Fu, Yaopeng [1 ]
Xie, Liping [1 ,2 ]
Zhang, Rongqing [1 ,2 ]
机构
[1] Tsinghua Univ, Inst Marine Biotechnold, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
[2] Tsinghua Univ, Prot Sci Lab, Minist Educ, Beijing 100084, Peoples R China
基金
中国国家自然科学基金;
关键词
37 kDa laminin receptor precursor; mantle epithelial cell; pearl oyster;
D O I
10.1016/j.pnsc.2007.06.009
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
A 1063 by cDNA clone encoding a putative 37 kDa laminin receptor precursor (37 kDa LRP) is isolated from the mantle tissue of pearl oyster, Pinctada fucata. The amino acid sequence predicted from the cDNA sequence is 301 residues long, with a calculated molecular mass of 33.5 kDa. RT-PCR analysis shows that 37 kDa LRP mRNA is especially highly expressed in the mantle while widely expressed in several tissues. In situ hybridization analysis reveals that 37 kDa LRP is expressed in the outer epithelial cells of the mantle edge, suggesting its involvement in cell proliferation and secretion in P. fucata. The identification and characterization of 37 kDa LRP in the pearl oyster will help us to further understand the signal transduction in the processes of mantle epithelial cell proliferation and tissue formation. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
引用
收藏
页码:233 / 238
页数:6
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