Kinetic behaviour in supercritical fluid chromatography with modified mobile phase for 5 μm particle size and varied flow rates

被引:27
作者
Lesellier, E. [1 ]
Fougere, L. [1 ]
Poe, Donald P. [2 ]
机构
[1] ICOA, UFR Sci, UMR 6005, F-45067 Orleans 2, France
[2] Univ Minnesota, Dept Chem & Biochem, Duluth, MN 55812 USA
关键词
Supercritical fluid chromatography; Efficiency; Particle size; High flow rate; Temperature gradient; Kinetic plots; ULTRA-HIGH-PRESSURE; LIQUID-CHROMATOGRAPHY; HIGH-TEMPERATURE; CARBON-DIOXIDE; PLOT METHOD; DISTRIBUTION ISOTHERMS; PERFORMANCE; EFFICIENCY; COLUMN; SYSTEMS;
D O I
10.1016/j.chroma.2010.12.057
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
After much development of stationary phase chemistry, in recent years the focus of many studies in HPLC has shifted to increase the efficiency and analysis speed. Ultra high pressure liquid chromatography (UHPLC) using sub-2 mu m particles, and high temperature liquid chromatography (HTLC), using temperatures above 100 degrees C have received much attention. These new approaches allow the use of flow rates higher than those classically used in HPLC, reducing the analysis duration. Due to the low viscosity of supercritical fluids, high velocities, i.e. high flow rates, can be achieved with classical pumping systems typically used in supercritical fluid chromatography (SFC). The effects of the flow rate increase with CO2/methanol mobile phase was studied on the inlet pressure, t(0), the retention factor of the compounds, and on the efficiency. Simple comparisons of efficiencies obtained at varied temperature between SFC and HPLC, with a packed column containing 5 mu m particles, show the greater kinetic performances achieved with the CO2/methanol fluid, and underline specific behaviours of SFC, occurring for high flow rates and sub-ambient temperature. Some values (N/t(0)) are also compared to UHPLC data, showing that good performance can be achieved in SFC without applying drastic analytical conditions. Finally, simple kinetic plots (t(0) vs N) at constant column length are used to select combinations of temperature and flow rate necessary to achieve a required theoretical plate number. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:2058 / 2064
页数:7
相关论文
共 37 条
[1]   THE EFFECT OF ADSORBED MOBILE-PHASE COMPONENTS ON THE RETENTION MECHANISM, EFFICIENCY, AND PEAK DISTORTION IN SUPERCRITICAL-FLUID CHROMATOGRAPHY [J].
BERGER, TA .
CHROMATOGRAPHIA, 1993, 37 (11-12) :645-652
[2]   EFFICIENCY LOSSES CAUSED BY GRADIENTS IN SOLUTE VELOCITY IN PACKED-COLUMN SUPERCRITICAL-FLUID CHROMATOGRAPHY [J].
BERGER, TA ;
BLUMBERG, LM .
CHROMATOGRAPHIA, 1994, 38 (1-2) :5-11
[3]   EFFICIENCY IN PACKED-COLUMN SUPERCRITICAL FLUID CHROMATOGRAPHY USING A MODIFIED MOBILE PHASE [J].
BERGER, TA ;
DEYE, JF .
CHROMATOGRAPHIA, 1991, 31 (11-12) :529-534
[4]   Relation between the particle size distribution and the kinetic performance of packed columns -: Application to a commercial sub-2 μm particle material [J].
Billen, Jeroen ;
Guillarme, Davy ;
Rudaz, Serge ;
Veuthey, Jean-Luc ;
Ritchie, Harald ;
Grady, Brian ;
Desmet, Gert .
JOURNAL OF CHROMATOGRAPHY A, 2007, 1161 (1-2) :224-233
[5]   Use of the kinetic plot method to analyze commercial high-temperature liquid chromatography systems I: Intrinsic performance comparison [J].
Cabooter, D. ;
Heinisch, S. ;
Rocca, J. L. ;
Clicq, D. ;
Desmet, G. .
JOURNAL OF CHROMATOGRAPHY A, 2007, 1143 (1-2) :121-133
[6]   Kinetic plot and particle size distribution analysis to discuss the performance limits of sub-2 μm and supra-2 μm particle columns [J].
Cabooter, Deirdre ;
Billen, Jeroen ;
Terryn, Herman ;
Lynen, Frederic ;
Sandra, Pat ;
Desmet, Gert .
JOURNAL OF CHROMATOGRAPHY A, 2008, 1204 (01) :1-10
[7]   Kinetic plot method as a tool to design coupled column systems producing 100,000 theoretical plates in the shortest possible time [J].
Cabooter, Deirdre ;
Lestremau, Francois ;
Lynen, Frederic ;
Sandra, Pat ;
Desmet, Gert .
JOURNAL OF CHROMATOGRAPHY A, 2008, 1212 (1-2) :23-34
[8]   Use of the kinetic plot method to analyze commercial high-temperature liquid chromatography systems - II. Practically constrained performance comparison [J].
Clicq, D. ;
Heinisch, S. ;
Rocca, J. L. ;
Cabooter, D. ;
Gzil, P. ;
Desmet, G. .
JOURNAL OF CHROMATOGRAPHY A, 2007, 1146 (02) :193-201
[9]   Influence of frictional heating on temperature gradients in ultra-high-pressure liquid chromatography on 2.1 mm ID columns [J].
de Villiers, A ;
Lauer, H ;
Szucs, R ;
Goodall, S ;
Sandra, P .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1113 (1-2) :84-91
[10]   Evaluation of ultra performance liquid chromatography -: Part I.: Possibilities and limitations [J].
de Villiers, Andre ;
Lestremau, Francois ;
Szucs, Roman ;
Gelebart, Sylvie ;
David, Frank ;
Sandra, Pat .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1127 (1-2) :60-69