Intelligent bio-assembly imaging-guided platform or real-time bacteria sterilizing and infectious therapy

被引：16

作者：

Zeng, Jiayu ^{[1
]}

Guo, Zengchao ^{[1
]}

Wang, Yihan ^{[1
]}

Qin, Zhaojian ^{[1
]}

Ma, Yi ^{[3
]}

Jiang, Hui ^{[1
]}

Weizmann, Yossi ^{[2
]}

Wang, Xuemei ^{[1
]}

机构：

[1] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Chien Shiung Wu Lab, Nanjing 210096, Peoples R China

[2] Ben Gurion Univ Negev, Dept Chem, IL-8410501 Beer Sheva, Israel

[3] China Pharmaceut Univ, Dept Engn, Nanjing 211198, Peoples R China

来源：

NANO RESEARCH | 2022年 / 15卷 / 05期

基金：

中国国家自然科学基金;

关键词：

bacterial microenvironment response; self-assembled Fe@Au-DNA complexes; bacterial fluorescence bioimaging; bacteria inactivation; wound healing; GOLD NANOCLUSTERS; AU NANOPARTICLES; FLUORESCENT; ANTIBACTERIAL; COMPLEXES; BIOSENSOR; LIGHT;

D O I：

10.1007/s12274-021-3998-3

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Bacterial infection is rising as a threatening health issue. Because of the present delay in early diagnosis of bacterial diseases as well as the abuse of antibiotics, it has become a vital issue in the development of in-time detection and therapy of bacterial infections. Herein, we designed a multifunctional nanotheranostics platform based on the unique micro-environment of bacterial infections to achieve specific bioimaging and simultaneous inactivation of the target bacteria. We showed that in bacterial infections, the metal precursors (i.e., HAuCl4, FeCl2, and herring sperm DNA) could be readily bio-self-assembled to multifunctional nanoclusters (NCs) that exhibit luminescence, in which AuCl4- was biosynthesized via reductive biomolecules such as NADPH to the fluorescent AuNCs. The DNA may assist as an encapsulation and delivery vector, and Fe2+ served as a fluorescence intensifier and reduced reactive oxygen species (ROS) to produce the iron oxides. While the bacteria were being visualized, the microenvironment-responsive NCs were enabled to sterilize bacteria efficiently due to electrostatic effect, cell membrane destruction, inhibition of biofilm formation, and ROS accumulation. Besides, the bio-responsive self-assembled NCs complexes contributed to accelerating bacteria-infected wound healing and showed negligible side effects in long-term toxicity tests in vivo. Also, intracellular molecules involved in microenvironmental response were investigated. The work may become an effective strategy for the detection and real-time sterilization of intractable bacterial infections.

引用

页码：4164 / 4174

页数：11