Nanoencapsulation of Hirudo medicinalis proteins in liposomes as a nanocarrier for inhibiting angiogenesis through targeting VEGFA in the Breast cancer cell line (MCF-7)
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Shakouri, Amir
[1
,2
]
Kahroba, Houman
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Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Mol Med, Tabriz, IranTabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran
Kahroba, Houman
[2
]
Hamishekar, Hamed
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Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, IranTabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran
Hamishekar, Hamed
[1
]
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Abdolalizadeh, Jalal
[3
,4
]
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[1] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran
[2] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Mol Med, Tabriz, Iran
[3] Tabriz Univ Med Sci, Immunol Res Ctr, Tabriz, Iran
[4] Tabriz Univ Med Sci, Paramed Fac, Tabriz, Iran
Introduction: Breast cancer is the most serious cause of women's death throughout the world. Using nanocarrier vehicles to the exact site of cancer upgrades the therapeutic efficiency of the drugs. Capsulation of active proteins in the vesicular liposomes' hydrophilic core is essential to develop a therapeutic protein carrier system. We aimed to encapsulate the medicinal leech saliva extract (LSE) and assess the inhibition of angiogenesis of breast cancer cells by targeting vascular endothelial growth factor A (VEGFA). Methods: In this research, enhanced formulation of liposomal protein was determined by zeta potential analysis, droplet size, drug release assay, and transmission electron microscopy (TEM). Furthermore, a cytotoxicity assay of liposomal LSE was performed to determine the cytotoxic activity of components. For assessing the expression of VEGFA, P53, and hypoxia-inducible factor subunit alpha (HIF1a) genes, Real-Time PCR was applied. Results: Nano liposome was chosen as an enhanced formulation due to its much smaller size (46.23 nm). Liposomal LSE had more practical actions on the MCF-7 cells. As noticed by DAPI staining, apoptosis was extensively greater in treated MCF-7 cells. Wound healing assay demonstrated that MCF-7 cells could not sustain growth at the presence of liposomal LSE and expression of the VEGFA gene was declined in treated cells. Downregulation of VEGFA was evaluated with western blotting technique. Conclusion: It can be concluded that our investigation of the tests confirmed the fact that nano liposomal LSE is a novel promising formulation for anticancer drugs and can significantly improve the penetration of protein drugs to cancer cells.
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Tabriz Univ Med Sci, Fac Pharm, Res Ctr Pharmaceut Nanotechnol, Tabriz, Iran
Tabriz Univ Med Sci, Sch Adv Biomed Sci, Tabriz, IranTabriz Univ Med Sci, Fac Pharm, Res Ctr Pharmaceut Nanotechnol, Tabriz, Iran
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Univ Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, AustraliaUniv Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, Australia
Belfiore, Lisa
Saunders, Darren N.
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Univ New South Wales, Sch Med Sci, Sydney, NSW, AustraliaUniv Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, Australia
Saunders, Darren N.
Ranson, Marie
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Univ Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, AustraliaUniv Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, Australia
Ranson, Marie
Thurecht, Kristofer J.
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Univ Queensland, Australian Res Council, Ctr Excellence Convergent Bionano Sci & Technol, AIBN,CAI, Brisbane, Qld, AustraliaUniv Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, Australia
Thurecht, Kristofer J.
Storm, Gert
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Univ Utrecht, Utrecht Inst Pharmaceut Sci, Dept Pharmaceut, Utrecht, NetherlandsUniv Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, Australia
Storm, Gert
Vine, Kara L.
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Univ Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, AustraliaUniv Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, Australia
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Tabriz Univ Med Sci, Fac Pharm, Res Ctr Pharmaceut Nanotechnol, Tabriz, Iran
Tabriz Univ Med Sci, Sch Adv Biomed Sci, Tabriz, IranTabriz Univ Med Sci, Fac Pharm, Res Ctr Pharmaceut Nanotechnol, Tabriz, Iran
机构:
Univ Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, AustraliaUniv Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, Australia
Belfiore, Lisa
Saunders, Darren N.
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Univ New South Wales, Sch Med Sci, Sydney, NSW, AustraliaUniv Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, Australia
Saunders, Darren N.
Ranson, Marie
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Univ Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, AustraliaUniv Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, Australia
Ranson, Marie
Thurecht, Kristofer J.
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Univ Queensland, Australian Res Council, Ctr Excellence Convergent Bionano Sci & Technol, AIBN,CAI, Brisbane, Qld, AustraliaUniv Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, Australia
Thurecht, Kristofer J.
Storm, Gert
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Univ Utrecht, Utrecht Inst Pharmaceut Sci, Dept Pharmaceut, Utrecht, NetherlandsUniv Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, Australia
Storm, Gert
Vine, Kara L.
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Univ Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, AustraliaUniv Wollongong, Sch Biol Sci, Ctr Med & Mol Biosci, Illawarra Hlth & Med Res Inst, Wollongong, NSW, Australia