Blepharisides A and B, new flavonol glycosides from Blepharis ciliaris growing in Saudi Arabia

被引：21

作者：

Mohamed, Gamal A. ^{[1
,2
]}

Ibrahim, Sabrin R. M. ^{[3
,4
]}

Elkhayat, Ehab S. ^{[2
]}

Ross, Samir A. ^{[5
]}

Sayed, Hanaa M. ^{[4
]}

El-Moghazy, Safaa A. M. ^{[4
]}

El-Shanawany, Mohamed A. ^{[4
]}

机构：

[1] King Abdulaziz Univ, Dept Nat Prod & Alternat Med, Fac Pharm, Jeddah 21589, Saudi Arabia

[2] Al Azhar Univ, Assiut Branch, Dept Pharmacognosy, Fac Pharm, Assiut 71524, Egypt

[3] Taibah Univ, Dept Pharmacognosy & Pharmaceut Chem, Fac Pharm, Al Madinah Al Munawwarah 30078, Saudi Arabia

[4] Assiut Univ, Dept Pharmacognosy, Fac Pharm, Assiut 71526, Egypt

[5] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, Natl Ctr Nat Prod Res, University, MS 38677 USA

来源：

PHYTOCHEMISTRY LETTERS | 2015年 / 11卷

关键词：

Blepharis ciliaris; Acanthaceae; Blepharisides; Hepatoprotective; Antioxidant; Anti-inflammatory; ANTIOXIDANT;

D O I：

10.1016/j.phytol.2014.12.018

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Bio-guided fractionation of the total MeOH extract (TME) of the aerial parts of Blepharis ciliaris (L.) B.L. Burtt. (Acanthaceae) growing in Saudi Arabia was carried out to evaluate its hepatoprotective activity against CCl4-induced hepatotoxicity in rats. Successive chromatographic separations of the potent hepatoprotective n-BuOH fraction afforded two new flavonol glycosides, blepharisides A (1) and B (2), along with quercetin 3-O-rutinoside (3). Their structures were established by UV, IR, 1D, 2D NMR, and HRESIMS spectral data, in addition to comparison with literature data. Co-treatment of CCl4 hepatic injured rats with the total MeOH extract (TME) and its fractions significantly restored the hepatic marker enzymes and total bilirubin to near-normal values compared to silymarin (reference drug). The isolated compounds were evaluated for their antioxidant and anti-inflammatory activities. They displayed significant antioxidant activity (DPPH assay) in relation to propyl gallate (positive control) (% inhibition of 88.2, 87.9, and 74.2, respectively). Compounds 1 and 2 demonstrated anti-inflammatory effects in the carrageenin induced paw edema method at a dose of 10 mg/kg. (C) 2014 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.

引用

页码：177 / 182

页数：6

共 29 条

[1] A novel 4′-O-diglycoside of decarboxyrosmarinic acid from Blepharis ciliaris [J].