Electrochemical genosensors for genetically modified organisms (GMOs) detection

被引：0
作者：
Carpini, G [1 ]
Marrazza, G [1 ]
Mascini, M [1 ]
机构：
[1] Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, FI, Italy
来源：
SENSORS AND MICROSYSTEMS, PROCEEDINGS | 2004年
关键词：
D O I：
10.1142/9789812702944_0011
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
A new disposable electrochemical genosensor for the detection of characteristic sequences related to genetically modified organisms (GMOs) was developed. Thiol modified oligonucleotides were immobilised onto gold screen-printed electrodes by self-assembled monolayer technique. The genosensors were hybridised with biotinylated complementary sequences. Streptavidin-alkaline phosphatase was coupled to the hybrid through streptavidin-biotin bond and the electroactive product of enzymatic hydrolysis, a-naphthol, could be detected by differential pulse voltammetry (DPV). The detection limit was 1 mu g/L.
引用
收藏
页码：82 / 87
页数：6
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