Transforming growth factor beta 1 mediates the low-frequency vertical vibration enhanced production of tenomodulin and type I collagen in rat Achilles tendon

被引：9

作者：

Chen, Chia-Hsin ^{[1
,2
,3
]}

Lin, Yi-Hsiung ^{[3
,4
,5
]}

Chen, Chung-Hwan ^{[3
,6
,7
,8
]}

Wang, Yan-Hsiung ^{[3
,9
,10
]}

Yeh, Ming-Long ^{[11
]}

Cheng, Tsung-Lin ^{[3
,4
,10
]}

Wang, Chau-Zen ^{[3
,4
,5
,10
]}

机构：

[1] Kaohsiung Med Univ Hosp, Dept Phys Med & Rehabil, Kaohsiung, Taiwan

[2] Kaohsiung Med Univ, Coll Med, Fac Med, Dept Phys Med & Rehabil, Kaohsiung, Taiwan

[3] Kaohsiung Med Univ, Orthopaed Res Ctr, Kaohsiung, Taiwan

[4] Kaohsiung Med Univ, Coll Med, Dept Physiol, Kaohsiung, Taiwan

[5] Kaohsiung Med Univ, Coll Med, Grad Inst Med, Kaohsiung, Taiwan

[6] Kaohsiung Med Univ, Coll Med, Dept Orthopaed, Kaohsiung, Taiwan

[7] Kaohsiung Med Univ, Kaohsiung Municipal Ta Tung Hosp, Dept Orthoped, Kaohsiung, Taiwan

[8] Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Orthoped, Div Adult Reconstruct Surg, Kaohsiung, Taiwan

[9] Kaohsiung Med Univ, Sch Dent, Kaohsiung, Taiwan

[10] Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Med Res, Kaohsiung, Taiwan

[11] Natl Cheng Kung Univ, Dept Biomed Engn, 1 Univ Rd, Tainan, Taiwan

来源：

PLOS ONE | 2018年 / 13卷 / 10期

关键词：

WHOLE-BODY VIBRATION; HUMAN PATELLAR TENDON; GENE-EXPRESSION; MECHANICAL-PROPERTIES; HORMONAL RESPONSES; SKELETAL-MUSCLE; EXERCISE; MATRIX; STRENGTH; DECORIN;

D O I：

10.1371/journal.pone.0205258

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Vertical vibration (VV) is a whole-body vibration with mechanical loading that commonly used in rehabilitation and sports training to increase athlete muscle strength. Our previous study showed that low-magnitude, low-frequency VV at 8 Hz and 10 Hz increased myoblast myogenesis. Herein, we investigated whether a VV frequency at low-frequency 5-10 Hz has anabolic effects on tenocytes and improves tendon stiffness. In primary tenocytes, 10 Hz VV treatment increased the tenogenic marker gene expression of tenomodulin and extracellular matrix type I collagen but decreased decorin expression. qPCR and Enzyme-Linked Immunosorbent Assay (ELISA) results showed that TGF-beta 1 expression was increased in tenocytes after 3 days of 10 Hz VV treatment in vitro and in Achilles tendons after 3 weeks in vivo. Tenomodulin expression and Achilles tendon stiffness were significantly increased in Achilles tendons after 3 weeks in vivo. We also showed that the TGF-beta 1 receptor inhibitor SB431542 (10 mu M) decreased the expression of tenomodulin and type I collagen but increased the decorin expression in tenocytes. These results indicated that the 10 Hz VV stimulated anabolic effects in tenocytes by increasing TGF-beta 1 expression that subsequently increases the expression of tenomodulin and type I collagen, and increased the Achilles tendon stiffness. This study provides insight into the low-frequency 10 Hz VV treatment improves tendon properties and can minimizes the risk of ligament/tendon reinjure during rehabilitation.

引用

页数：19

共 51 条

[51] Decorin regulates assembly of collagen fibrils and acquisition of biomechanical properties during tendon development
Zhang, Guiyun
Ezura, Yoichi
Chervoneva, Inna
Robinson, Paul S.
Beason, David P.
Carine, Ehren T.
Soslowsky, Louis J.
Iozzo, Renato V.
Birk, David E.
[J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 2006, 98 (06) : 1436 - 1449

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