Ionic Liquid Enables Simple and Rapid Sample Preparation of Human Culturing Cells for Scanning Electron Microscope Analysis

被引：59

作者：

Ishigaki, Yasuhito

Nakamura, Yuka ^{[2
]}

Takehara, Teruaki

Nemoto, Noriko ^{[3
]}

Kurihara, Takayuki ^{[4
]}

Koga, Hironori ^{[4
]}

Nakagawa, Hideaki

Takegami, Tsutomu

Tomosugi, Naohisa ^{[1
,2
]}

Miyazawa, Shichiro ^{[3
]}

Kuwabata, Susumu ^{[5
,6
]}

机构：

[1] Kanazawa Med Univ, Div Adv Med, Med Res Inst, Uchinada, Ishikawa 9200293, Japan

[2] MCProt Biotechnol Co Ltd, Uchinada, Ishikawa 9200293, Japan

[3] Kitasato Univ, Sch Med, Res Ctr Biol Imaging, Kanagawa 2520374, Japan

[4] Ishikawa Prefectural Univ, Fac Bioresources & Environm Sci, Nonoichi, Ishikawa 9218836, Japan

[5] Osaka Univ, Grad Sch Engn, Dept Appl Chem, Suita, Osaka 5650871, Japan

[6] CREST, JST, Kawaguchi, Saitama 3320012, Japan

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2011年 / 74卷 / 05期

关键词：

1-ethyl-3-methyl-imidazolium tetrafluoroborate (EMI-BF4); SEM; A549; cell;

D O I：

10.1002/jemt.20924

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Ionic liquid is a kind of salt that stays in a molten state even at room temperature. It does not vaporize at all in vacuum and facilitates electrical conductivity to the sample surfaces for observations with a scanning electron microscope (SEM). In this study, we used an ionic liquid in SEM for the first time to observe fixed human culture cells. The condition for the cell culture using wrapping sheets and SEM settings were varied to elucidate the optimized protocol. Compared to samples prepared by the conventional way, the ionic liquid-treatment of samples gave SEM images of the cellular ultra structures in more detail, enabling observation of microvilli that made bridges between separated cells. In addition, the ionic liquid treatment is less time consuming as well as less laborious compared with the conventional way that includes dehydration, drying, and conductivity treatments. Totally, we concluded the ionic liquid is a useful reagent for SEM sample preparation. Microsc. Res. Tech. 74:415-420, 2011. (C) 2010 Wiley-Liss, Inc.

引用

页码：415 / 420

页数：6

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