Short report:: Polymorphisms in the pfcrt and pfmdr1 genes of Plasmodium falciparum and in vitro susceptibility to arnodiaquine and desethylarnodiaquine

被引：43
作者：
Echeverry, Diego F.
Holmgren, Gabrielle
Murillo, Claribel
Higuita, Juan C.
Bjorkman, Anders
Gil, Jose P.
Osorio, Lyda
机构：
[1] Int Ctr Med Res & Training CIDEIM, Cali, Colombia
[2] Karolinska Inst, Div Med, Dept Infect Dis, Malaria Res Unit, Stockholm, Sweden
来源：
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2007年 / 77卷 / 06期
关键词：
D O I：
10.4269/ajtmh.2007.77.1034
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
The potential role of polymorphisms in the pfcrt and pfmdr1 genes and in vitro susceptibility to amodiaquine and desethylamodiaquine were explored in 15 chloroquine-resistant Colombian Plasmodium falciparum isolates. Single nucleotide polymorphisms in the pfcrt gene, including a newly reported mutation (S334N), were seen in isolates with decreased susceptibility to amodiaquine and desethylamodiaquine. The lowest susceptibility found to amodiaquine was observed in an isolate carrying a pfcrt and pfmdr1 Dd2-like haplotype, whereas a pfcrt haplotype related to the 7G8 Brazilian strain was found in a Colombian isolate with the lowest susceptibility to desethylamodiaquine. This exploratory study suggests that polymorphisms in the pfcrt and pfmdr1 genes play a role in amodiaquine and desethylamodiaquine resistance and warrants further study.
引用
收藏
页码：1034 / 1038
页数：5
相关论文
共 31 条
[1]  
Adjuik M, 2004, LANCET, V363, P9, DOI 10.1016/S0140-6736(03)15162-8
[2]   Therapeutic efficacy test in malaria falciparum in Antioquia, Colombia [J].
Blair, S ;
Carmona-Fonseca, J ;
Piñeros, JG ;
Ríos, A ;
Alvarez, T ;
Alvarez, G ;
Tobón, A .
MALARIA JOURNAL, 2006, 5 (1)
[3]   Amodiaquine remains effective for treating uncomplicated malaria in West and Central Africa [J].
Brasseur, P ;
Guiguemde, R ;
Diallo, S ;
Guiyedi, V ;
Kombila, A ;
Ringwald, P ;
Olliaro, P .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1999, 93 (06) :645-650
[4]   Antimalarial drug susceptibility testing of Plasmodium falciparum in Brazil using a radioisotope method [J].
Cerutti, C ;
Marques, C ;
de Alencar, FEC ;
Durlacher, RR ;
Alween, A ;
Segurado, AAC ;
Pang, LW ;
Zalis, MG .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1999, 94 (06) :803-809
[5]   Sequence polymorphisms in pfcrt are strongly associated with chloroquine resistance in Plasmodium falciparum [J].
Chen, N ;
Russell, B ;
Staley, J ;
Kotecka, B ;
Nasveld, P ;
Cheng, Q .
JOURNAL OF INFECTIOUS DISEASES, 2001, 183 (10) :1543-1545
[6]   AMODIAQUINE AS A PRODRUG - IMPORTANCE OF METABOLITE(S) IN THE ANTIMALARIAL EFFECT OF AMODIAQUINE IN HUMANS [J].
CHURCHILL, FC ;
PATCHEN, LC ;
CAMPBELL, CC ;
SCHWARTZ, IK ;
PHUC, ND ;
DICKINSON, CM .
LIFE SCIENCES, 1985, 36 (01) :53-62
[7]   Origin and dissemination of Plasmodium falciparum drug-resistance mutations in South America [J].
Cortese, JF ;
Caraballo, A ;
Contreras, CE ;
Plowe, CV .
JOURNAL OF INFECTIOUS DISEASES, 2002, 186 (07) :999-1006
[8]   QUANTITATIVE ASSESSMENT OF ANTI-MALARIAL ACTIVITY INVITRO BY A SEMIAUTOMATED MICRODILUTION TECHNIQUE [J].
DESJARDINS, RE ;
CANFIELD, CJ ;
HAYNES, JD ;
CHULAY, JD .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1979, 16 (06) :710-718
[9]  
Djimdé A, 2001, NEW ENGL J MED, V344, P257, DOI 10.1056/NEJM200101253440403
[10]   Variations in the sequence and expression of the Plasmodium falciparum chloroquine resistance transporter (Pfcrt) and their relationship to chloroquine resistance in vitro [J].
Durrand, V ;
Berry, A ;
Sem, R ;
Glaziou, P ;
Beaudou, J ;
Fandeur, T .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2004, 136 (02) :273-285
1234