Lectin purified from Musca domestica pupa up-regulates NO and iNOS production via TLR4/NF-κB signaling pathway in macrophages

被引：17

作者：

Cao, Xiaohong ^{[1
]}

Zhou, Minghui ^{[1
]}

Wang, Chunling ^{[1
]}

Hou, Lihua ^{[1
]}

Zeng, Bin ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, Key Lab Food Safety & Sanitat, Tianjin Econ Technol Dev Area,Minist Educ, Tianjin 300457, Peoples R China

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2011年 / 11卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Lectin; Musca domestica pupa; NO; iNOS; NF-kappa B; NF-KAPPA-B; GALACTOSE-BINDING LECTIN; ANTITUMOR ACTIVITIES; PLANT-LECTINS; KINASE; INHIBITION; EXPRESSION; INDUCTION; MUSHROOM; GROWTH;

D O I：

10.1016/j.intimp.2010.12.004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The present study reported that nitric oxide (NO) was up-regulated by the induction of lectin purified from Musca domestica pupa (MPL) in macrophages without cytotoxicity. The mRNA expression and protein secretion of inducible nitric oxide synthase (iNOS) were strongly induced by MPL treatments. Subsequent investigation revealed that the nuclear factor-kappa B (NF-kappa B) inhibitory kappa B (I kappa B) in endochylema was inhibited and NF-kappa B translocated into the nucleus after MPL treatment. Meanwhile, the IKK beta was strongly induced and the production of the toll-like receptor 4 (TLR4) was significantly up- regulated. Moreover, MPL increased NO production via inducing the expression of iNOS through the activation of NF-kappa B, which suggested that MPL probably acted as an activating agent of the NF-kappa B activation. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：399 / 405

页数：7

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