Detection of metallo-β-lactamase-producing Pseudomonas aeruginosa strains isolated from burn patients in Ahwaz, Iran

被引：66
|
作者：
Khosravi, Azar D. [1 ,2 ]
Mihani, Fatemeh [1 ]
机构：
[1] Ahwaz Jondishapour Univ Med Sci, Dept Microbiol, Sch Med, Ahvaz, Iran
[2] Ahwaz Jondishapour Univ Med Sci, Infect & Trop Dis Res Ctr, Ahvaz, Iran
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 60卷 / 01期
关键词：
metallo-beta-lactamase; P; aeruginosa; bum patient; etest; PCR;
D O I：
10.1016/j.diagmicrobio.2007.08.003
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The antimicrobial activities of 6 common antimicrobial agents including carbapenems were tested against 100 clinical Pseudomonas aeruginosa isolates using a disk diffusion method. All imipenem (IPM)-resistant isolates were screened for metallo-beta-lactamase (MBL) production by Etest assay and were subsequently subjected to polymerase chain reaction (PCR) analysis with the bla(IMP) and bla(VIM) genes. Of 41 IPM-nonsusceptible isolates detected, 8 (19.51%) appeared to produce MBL, as determined by Etest. Using PCR assay, these isolates were positive for bla(VIM) genes, whereas none were positive for bla(IMP) genes. (c) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：125 / 128
页数：4
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