Production, preliminary characterization and antitumor activity in vitro of polysaccharides from the mycelium of Pholiota dinghuensis Bi

被引：108
作者：
Gan, Dan [1 ,2 ]
Ma, Liping [1 ]
Jiang, Changxing [1 ]
Xu, Renjie [1 ]
Zeng, Xiaoxiong [1 ]
机构：
[1] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China
[2] Xuzhou Coll Ind Technol, Dept Chem Engn, Xuzhou 221140, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2011年 / 84卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Pholiota dinghuensis Bi; Polysaccharide; Production; Preliminary characterization; Antitumor activity; SUBMERGED CULTURE-CONDITIONS; GRIFOLA-FRONDOSA; OPTIMIZATION; EXOPOLYSACCHARIDES; PURIFICATION; EXTRACTION; NAMEKO;
D O I：
10.1016/j.carbpol.2010.12.058
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The production, preliminary characterization and antitumor activity in vitro of mycelial polysaccharides from Pholiota dinghuensis Bi (PDP) were investigated in the present study. Firstly, crude PDP was prepared from the mycelia of P. dinghuensis Bi by submerged culture. Then, the crude PDP was purified by chromatography of DEAE-52 cellulose and Sephadex G-100, resulting three purified fractions of PDP-1. PDP-2 and PDP-3. We found that the monosaccharide composition of PDP-3 was greatly different from that of PDP-1 or PDP-2. In addition, it contained the highest contents of protein, sulfate and uronic acid among the polysaccharides tested. Furthermore, PDP-3 exhibited higher antiproliferative activity against human gastric cancer BGC-823 cells in vitro than crude PDP. PDP-1 or PDP-2. At a concentration of 400 mg/L and an exposure time of 72 h, the inhibition rates for crude PDP, PDP-2 and PDP-3 were 69.36%, 73.65% and 85.78%, respectively. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：997 / 1003
页数：7
相关论文
共 50 条
[31]   Preparation, identification and their antitumor activities in vitro of polysaccharides from Chlorella pyrenoidosa [J].
Sheng, Jlanchun ;
Yu, Fang ;
Xin, Zhihong ;
Zhao, Liyan ;
Zhu, Xiaojun ;
Hu, Qiuhui .
FOOD CHEMISTRY, 2007, 105 (02) :533-539
[32]   Alkali extraction and in vitro antioxidant activity of Monascus mycelium polysaccharides [J].
Wang, Pengrong ;
Chen, Danfeng ;
Jiang, Donghua ;
Dong, Xiameng ;
Chen, Panpan ;
Lin, Yaoxue .
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, 2014, 51 (07) :1251-1259
[33]   Purification, characterization and immunomodulatory activity of polysaccharides from Leccinum crocipodium (Letellier.) Watliag [J].
Zheng, Tingting ;
Gu, Dahai ;
Wang, Xuefeng ;
Shen, Xiaojing ;
Yan, Liang ;
Zhang, Wenjie ;
Pu, Yuehong ;
Ge, Changrong ;
Fan, Jiangping .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 148 :647-656
[34]   Extraction optimization, preliminary characterization and immunological activity of polysaccharides from figs [J].
Chen, Ruizhan ;
Li, Haiping ;
Li, Shizhe ;
Jin, Chenguang ;
Lu, Juan .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2015, 72 :185-194
[35]   Structure and antitumor activities of the water-soluble polysaccharides from Ganoderma tsugae mycelium [J].
Peng, YF ;
Zhang, LN ;
Zeng, FB ;
Kennedy, JF .
CARBOHYDRATE POLYMERS, 2005, 59 (03) :385-392
[36]   PRODUCTION, PRELIMINARY CHARACTERIZATION, AND BIOACTIVITIES OF EXOPOLYSACCHARIDES FROM Pleurotus geesteranus 5 # [J].
He, Peixin ;
Geng, Lujing ;
Mao, Duobin ;
Wang, Zheng ;
Xu, Chunping .
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2013, 43 (01) :108-122
[37]   The structural characterization of a polysaccharide exhibiting antitumor effect from Pholiota adiposa mycelia [J].
Zou, Yajie ;
Du, Fang ;
Hu, Qingxiu ;
Wang, Hexiang .
SCIENTIFIC REPORTS, 2019, 9 (1)
[38]   Structural characterization of polysaccharides from Dictyophora rubrovolvata mycelium and their immunostimulatory activity in RAW264.7 cells [J].
Wang, Jiawen ;
Cheng, Xiaolei ;
Li, Tengda ;
Song, Mingyang ;
Wang, Siqiang ;
Wen, Tingchi ;
Zhu, Zhenyuan .
PROCESS BIOCHEMISTRY, 2024, 139 :22-32
[39]   Optimized Extraction, Preliminary Characterization, and In Vitro Antioxidant Activity of Polysaccharides from Glycyrrhiza Uralensis Fisch [J].
Chen, Jie ;
Li, Wan-chen ;
Gu, Xin-li .
MEDICAL SCIENCE MONITOR, 2017, 23 :1783-1791
[40]   PRELIMINARY STRUCTURAL CHARACTERIZATION, ANTIOXIDANT AND ANTITUMOR ACTIVITIES OF CHINESE CHESTNUT (CASTANEA MOLLISSIMA BLUME) POLYSACCHARIDES [J].
Wang, Tong-Kun ;
Du, Bin ;
Yang, Yue-Dong ;
Liang, Xue ;
Wang, Xiao-Hong ;
Zhou, Yan .
CURRENT TOPICS IN NUTRACEUTICAL RESEARCH, 2015, 13 (04) :241-248
12345