In vitro synergistic antibacterial activity of the essential oil from Zingiber cassumunar Roxb against extensively drug-resistant Acinetobacter baumannii strains

被引:45
作者
Boonyanugomol, Wongwarut [1 ]
Kraisriwattana, Kairin [1 ]
Rukseree, Kamolchanok [1 ]
Boonsam, Kraisorn [2 ]
Narachai, Panchaporn [2 ]
机构
[1] Mahidol Univ, Amnat Chareon Campus, Amnat Chareon 37000, Thailand
[2] Amnat Chareon Hosp, Div Clin Pathol, Microbiol Lab, Amnat Chareon 37000, Thailand
关键词
Acinetobacter baumannii; Z; cassumunar; Essential oil; Antibacterial activity; Synergism; ANTIMICROBIAL ACTIVITY; ANTIOXIDANT; EXTRACTS;
D O I
10.1016/j.jiph.2017.01.008
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
In this study, we determined the antibacterial and synergistic activities of the essential oil from Zingiber cassumunar against the extensively drug-resistant (XDR) Acinetobacter baumannii strains. The antibacterial and synergistic properties of the essential oil from Z. cassumunar were examined by agar disc diffusion tests. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated by broth microdilution using the resazurin assay. The in vitro time-kill antibacterial kinetics was analyzed using the plate count technique. We found that the essential oil from Z. cassumunar had antibacterial activity against A. baumannii, with MIC and MBC ranging from 7.00 to 9.24 mg/ml. The essential oil could completely inhibit A. baumannii at 1 h, and coccoid-shaped bacteria were found after treatment. In addition, the essential oil had a synergistic effect when combined with antibiotics, e.g., aminoglycosides, fluoroquinolones, tetracyclines, and folate pathway inhibitors. Thus, the essential oil from Z. cassumunar has strong antibacterial and synergistic activities against XDR A. baumannii, which may provide the basis for the development of a new therapy against drug-resistant bacteria. (C) 2017 The Authors. Published by Elsevier Limited.
引用
收藏
页码:586 / 592
页数:7
相关论文
共 30 条
[1]  
[Anonymous], 2014, PERF STAND ANT SUSC
[2]  
[Anonymous], 2000, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that grow Aerobically, V5th
[3]  
Avila JG, 1999, J ETHNOPHARMACOL, V66, P75, DOI 10.1016/S0378-8741(98)00203-7
[4]   Biological effects of essential oils - A review [J].
Bakkali, F. ;
Averbeck, S. ;
Averbeck, D. ;
Waomar, M. .
FOOD AND CHEMICAL TOXICOLOGY, 2008, 46 (02) :446-475
[5]  
Bayala B, 2014, AM J CANCER RES, V4, P591
[6]  
Bin JI, 2003, PHARM BIOL, V41, P392
[7]  
Cb S., 2015, J. Pharmacogn. Phytochem, V4, P1
[8]   ACTIVE COMPOUND OF ZINGIBER CASSUMUNAR ROXB. DOWN-REGULATES THE EXPRESSION OF GENES INVOLVED IN JOINT EROSION IN A HUMAN SYNOVIAL FIBROBLAST CELL LINE [J].
Chaiwongsa, Rujirek ;
Ongchai, Siriwan ;
Boonsing, Phorani ;
Kongtawelert, Prachya ;
Panthong, Ampai ;
Reutrakul, Vichai .
AFRICAN JOURNAL OF TRADITIONAL COMPLEMENTARY AND ALTERNATIVE MEDICINES, 2013, 10 (01) :40-48
[9]   Screening of Zingiberaceae extracts for antimicrobial and antioxidant activities [J].
Habsah, M ;
Amran, M ;
Mackeen, MM ;
Lajis, NH ;
Kikuzaki, H ;
Nakatani, N ;
Rahman, AA ;
Ghafar ;
Ali, AM .
JOURNAL OF ETHNOPHARMACOLOGY, 2000, 72 (03) :403-410
[10]  
Koontongkaew Sittichai, 2014, Int Sch Res Notices, V2014, P632608, DOI 10.1155/2014/632608