Molecular cloning, expression pattern and phylogenetic analysis of myosin light chain 2 gene from Antheraea pernyi: A potential marker for phylogenetic inference

被引：8

作者：

Liu, Lin ^{[1
]}

Wang, Hui-Ying ^{[1
]}

Jin, Hong-Yu ^{[1
]}

Wu, Song ^{[1
]}

Li, Yu-Ping ^{[1
]}

Liu, Yan-Qun ^{[1
,2
]}

Li, Xi-Sheng ^{[2
]}

Qin, Li ^{[1
]}

Wang, Zhen-Dong ^{[1
]}

机构：

[1] Shenyang Agr Univ, Coll Biosci & Biotechnol, Dept Sericulture, Shenyang 110866, Peoples R China

[2] Sericultural Inst Liaoning Prov, Fengcheng 118100, Peoples R China

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2010年 / 38卷 / 05期

关键词：

Antheraea pernyi; Myosin light chain 2; Gene cloning; Expression pattern; Phylogeny; CHINESE OAK SILKWORM; CA2+ BINDING; SEQUENCE; GENOME; IDENTIFICATION; EVOLUTION; PROTEIN;

D O I：

10.1016/j.bse.2010.09.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Myosin light chain 2 (MLC-2) gene was isolated and characterized from Antheraea pernyi, a well-known wild silkmoth. The isolated cDNA sequence is 905 bp in length with an open reading frame of 612 bp encoding a polypeptide of 203 amino acids. Semi-quantitative RT-PCR analysis showed that the MLC-2 gene was transcribed during four developmental stages (egg, larva, pupa, and moth), and present in all tissues tested. Alignment analysis revealed that the deduced protein sequence has over 95% identity to myosin light chain 2 of lepidopteran species, and 57-88% identity to other insect species, suggesting that insect MLC-2 proteins are highly conserved throughout evolution. The protein sequence was used to construct phylogenetic trees with other known vertebrate and invertebrate MLC-2 sequences, and the obtained trees demonstrated similar topology with the classical systematics, indicating the potential value of MLC-2 gene in phylogenetic, study. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：981 / 987

页数：7

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