Chemical analysis and anti-proliferative activity of Campania Thymus Vulgaris essential oil

被引：21

作者：

Catauro, Michelina ^{[1
]}

Bollino, Flavia ^{[1
]}

Tranquillo, Elisabetta ^{[1
]}

Sapio, Luigi ^{[2
]}

Illiano, Michela ^{[2
]}

Caiafa, Ilaria ^{[2
]}

Naviglio, Silvio ^{[2
]}

机构：

[1] Univ Naples 2, Dept Ind & Informat Engn, Aversa, Italy

[2] Univ Naples 2, Sch Med, Dept Biochem Biophys & Gen Pathol, Naples, Italy

来源：

JOURNAL OF ESSENTIAL OIL RESEARCH | 2017年 / 29卷 / 06期

关键词：

Thymus vulgaris L; essential oil; anti-proliferative activity; human osteosarcoma cells; pancreatic cancer cells; IN-VITRO; ANTIMICROBIAL ACTIVITY; INORGANIC-PHOSPHATE; ANTITUMOR-ACTIVITY; DOWN-REGULATION; L; ANTIOXIDANT; PLANTS; GROWTH; WILD;

D O I：

10.1080/10412905.2017.1351405

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, the potential antitumor activity of an essential oil (EO) obtained from Thymus vulgaris L. plants cultivated in Campania (Southern Italy) was evaluated. The gas chromatography-mass spectrometry and Fourier transform infrared spectroscopy were used to investigate the chemical composition of EO. The monoterpenes thymol, p-cymene and carvacrol are the more abundant compounds detected. The anti-proliferative activity of the EO as a function of dose and time of exposure was evaluated by MTT assay on human osteosarcoma U2OS and pancreatic cancer PANC-1 cell lines. EO inhibits proliferation of both cell lines, but the effect on PANC-1 cells was more extensive. Flow cytometry and Western blot analyses revealed that the anti-proliferative effect is due to a decrease of cells in the S phase and their accumulation in the G1 phase, associated with the decrease of cyclin A and the increase of cell cycle inhibitor p27 protein levels, respectively.

引用

页码：461 / 470

页数：10

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