A guide to super-resolution fluorescence microscopy

被引：932
作者：
Schermelleh, Lothar [1 ,2 ]
Heintzmann, Rainer [3 ,4 ,5 ]
Leonhardt, Heinrich [1 ,2 ]
机构：
[1] Univ Munich, Dept Biol, D-82152 Planegg Martinsried, Germany
[2] Univ Munich, Ctr Integrated Prot Sci, D-82152 Planegg Martinsried, Germany
[3] Kings Coll London, Randall Div Cell & Mol Biophys, London SE1 1UL, England
[4] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany
[5] Inst Photon Technol, D-07745 Jena, Germany
来源：
JOURNAL OF CELL BIOLOGY | 2010年 / 190卷 / 02期
基金：
英国工程与自然科学研究理事会;
关键词：
STRUCTURED-ILLUMINATION MICROSCOPY; PATTERNED EXCITATION MICROSCOPY; GROUND-STATE-DEPLETION; HIGH-RESOLUTION; STIMULATED-EMISSION; DIFFRACTION-LIMIT; LOCALIZATION MICROSCOPY; LIGHT-MICROSCOPY; ADAPTIVE OPTICS; STED MICROSCOPY;
D O I：
10.1083/jcb.201002018
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new superresolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules. Overall, these new approaches have created unprecedented new possibilities to investigate the structure and function of cells.
引用
收藏
页码：165 / 175
页数：11
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