CRISPR-Cas12c: a noncleaving DNA binder with minimal PAM requirement

被引：5

作者：

Awan, Muhammad Jawad Akbar ^{[1
]}

Amin, Imran ^{[1
]}

Mansoor, Shahid ^{[1
]}

机构：

[1] Natl Inst Biotechnol & Genetc Engheerng NIBGE, Agr Biotechnol Div, Constituent Coll Pakistan Inst Engn & Appl Sci, Jhang Rd, Faisalabad, Pakistan

来源：

TRENDS IN BIOTECHNOLOGY | 2022年 / 40卷 / 10期

关键词：

D O I：

10.1016/j.tibtech.2022.07.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The CRISPR-Cas toolbox is ex-panding swiftly. Every discovery of a novel and unique variant opens new frontiers in the field of synthetic and applied biology. Recently, Huang et al. revealed the CRISPR-Cas12c system that requires a single-nucleotide protospacer ad-jacent motif (PAM) to perform RNA-guided DNA targeting with-out DNA cleavage.

引用

页码：1141 / 1143

页数：3

共 10 条

[1] CRISPR diagnostics
Abudayyeh, Omar O.
Gootenberg, Jonathan S.
[J]. SCIENCE, 2021, 372 (6545) : 914 - 915
[2] Bio-SCAN: A CRISPR/dCas9-Based Lateral Flow Assay for Rapid, Specific, and Sensitive Detection of SARS-CoV-2
Ali, Zahir
Sanchez, Edith
Tehseen, Muhammad
Mahas, Ahmed
Marsic, Tin
Aman, Rashid
Rao, Gundra Sivakrishna
Alhamlan, Fatimah Saeed
Alsanea, Madain S.
Al-Qahtani, Ahmed A.
Hamdan, Samir
Mahfouz, Magdy
[J]. ACS SYNTHETIC BIOLOGY, 2022, 11 (01): : 406 - 419
[3] Twin prime editor: seamless repair without damage
Awan, Muhammad Jawad Akbar
Ali, Zahir
Amin, Imran
Mansoor, Shahid
[J]. TRENDS IN BIOTECHNOLOGY, 2022, 40 (04) : 374 - 376
[4] A scoutRNA Is Required for Some Type V CRISPR-Cas Systems
Harrington, Lucas B.
Ma, Enbo
Chen, Janice S.
Witte, Isaac P.
Gertz, Dov
Paez-Espino, David
Al-Shayeb, Basem
Kyrpides, Nikos C.
Burstein, David
Banfield, Jillian F.
Doudna, Jennifer A.
[J]. MOLECULAR CELL, 2020, 79 (03) : 416 - +
[5] A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression
Huang, Carolyn J.
Adler, Benjamin A.
Doudna, Jennifer A.
[J]. MOLECULAR CELL, 2022, 82 (11) : 2148 - +
[6] Base editing: advances and therapeutic opportunities
Porto, Elizabeth M.
Komor, Alexis C.
Slaymaker, Ian M.
Yeo, Gene W.
[J]. NATURE REVIEWS DRUG DISCOVERY, 2020, 19 (12) : 839 - 859
[7] CRISPR-mediated live imaging of genome editing and transcription
Wang, Haifeng
Nakamura, Muneaki
Abbott, Timothy R.
Zhao, Dehua
Luo, Kaiwen
Yu, Cordelia
Nguyen, Cindy M.
Lo, Albert
Daley, Timothy P.
La Russa, Marie
Liu, Yanxia
Qi, Lei S.
[J]. SCIENCE, 2019, 365 (6459) : 1301 - +
[8] Prediction of biomarkers and therapeutic combinations for anti-PD-1 immunotherapy using the global gene network association
Wu, Chia-Chin
Wang, Y. Alan
Livingston, J. Andrew
Zhang, Jianhua
Futreal, P. Andrew
[J]. NATURE COMMUNICATIONS, 2022, 13 (01)
[9] Functionally diverse type V CRISPR-Cas systems
Yan, Winston X.
Hunnewell, Pratyusha
Alfonse, Lauren E.
Carte, Jason M.
Keston-Smith, Elise
Sothiselvam, Shanmugapriya
Garrity, Anthony J.
Chong, Shaorong
Makarova, Kira S.
Koonin, Eugene V.
Cheng, David R.
Scott, David A.
[J]. SCIENCE, 2019, 363 (6422) : 88 - +
[10] Engineering Plants for Geminivirus Resistance with CRISPR/Cas9 System
Zaidi, Syed Shan-e-Ali
Mansoor, Shahid
Ali, Zahir
Tashkandi, Manal
Mahfouz, Magdy M.
[J]. TRENDS IN PLANT SCIENCE, 2016, 21 (04) : 279 - 281

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