CRISPR-Cas12c: a noncleaving DNA binder with minimal PAM requirement

被引：6

作者：

Awan, Muhammad Jawad Akbar ^{[1
]}

Amin, Imran ^{[1
]}

Mansoor, Shahid ^{[1
]}

机构：

[1] Natl Inst Biotechnol & Genetc Engheerng NIBGE, Agr Biotechnol Div, Constituent Coll Pakistan Inst Engn & Appl Sci, Jhang Rd, Faisalabad, Pakistan

来源：

TRENDS IN BIOTECHNOLOGY | 2022年 / 40卷 / 10期

关键词：

D O I：

10.1016/j.tibtech.2022.07.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The CRISPR-Cas toolbox is ex-panding swiftly. Every discovery of a novel and unique variant opens new frontiers in the field of synthetic and applied biology. Recently, Huang et al. revealed the CRISPR-Cas12c system that requires a single-nucleotide protospacer ad-jacent motif (PAM) to perform RNA-guided DNA targeting with-out DNA cleavage.

引用

收藏

页码：1141 / 1143

页数：3

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共 10 条

[1] CRISPR diagnostics [J].

Abudayyeh, Omar O. ;

Gootenberg, Jonathan S. .

SCIENCE, 2021, 372 (6545) :914-915

[2] Bio-SCAN: A CRISPR/dCas9-Based Lateral Flow Assay for Rapid, Specific, and Sensitive Detection of SARS-CoV-2 [J].

Ali, Zahir ;

Sanchez, Edith ;

Tehseen, Muhammad ;

Mahas, Ahmed ;

Marsic, Tin ;

Aman, Rashid ;

Rao, Gundra Sivakrishna ;

Alhamlan, Fatimah Saeed ;

Alsanea, Madain S. ;

Al-Qahtani, Ahmed A. ;

Hamdan, Samir ;

Mahfouz, Magdy .

ACS SYNTHETIC BIOLOGY, 2022, 11 (01) :406-419

[3] Twin prime editor: seamless repair without damage [J].

Awan, Muhammad Jawad Akbar ;

Ali, Zahir ;

Amin, Imran ;

Mansoor, Shahid .

TRENDS IN BIOTECHNOLOGY, 2022, 40 (04) :374-376

[4] A scoutRNA Is Required for Some Type V CRISPR-Cas Systems [J].

Harrington, Lucas B. ;

Ma, Enbo ;

Chen, Janice S. ;

Witte, Isaac P. ;

Gertz, Dov ;

Paez-Espino, David ;

Al-Shayeb, Basem ;

Kyrpides, Nikos C. ;

Burstein, David ;

Banfield, Jillian F. ;

Doudna, Jennifer A. .

MOLECULAR CELL, 2020, 79 (03) :416-+

[5] A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression [J].

Huang, Carolyn J. ;

Adler, Benjamin A. ;

Doudna, Jennifer A. .

MOLECULAR CELL, 2022, 82 (11) :2148-+

[6] Base editing: advances and therapeutic opportunities [J].

Porto, Elizabeth M. ;

Komor, Alexis C. ;

Slaymaker, Ian M. ;

Yeo, Gene W. .

NATURE REVIEWS DRUG DISCOVERY, 2020, 19 (12) :839-859

[7] CRISPR-mediated live imaging of genome editing and transcription [J].

Wang, Haifeng ;

Nakamura, Muneaki ;

Abbott, Timothy R. ;

Zhao, Dehua ;

Luo, Kaiwen ;

Yu, Cordelia ;

Nguyen, Cindy M. ;

Lo, Albert ;

Daley, Timothy P. ;

La Russa, Marie ;

Liu, Yanxia ;

Qi, Lei S. .

SCIENCE, 2019, 365 (6459) :1301-+

[8] Prediction of biomarkers and therapeutic combinations for anti-PD-1 immunotherapy using the global gene network association [J].

Wu, Chia-Chin ;

Wang, Y. Alan ;

Livingston, J. Andrew ;

Zhang, Jianhua ;

Futreal, P. Andrew .

NATURE COMMUNICATIONS, 2022, 13 (01)

[9] Functionally diverse type V CRISPR-Cas systems [J].

Yan, Winston X. ;

Hunnewell, Pratyusha ;

Alfonse, Lauren E. ;

Carte, Jason M. ;

Keston-Smith, Elise ;

Sothiselvam, Shanmugapriya ;

Garrity, Anthony J. ;

Chong, Shaorong ;

Makarova, Kira S. ;

Koonin, Eugene V. ;

Cheng, David R. ;

Scott, David A. .

SCIENCE, 2019, 363 (6422) :88-+

[10] Engineering Plants for Geminivirus Resistance with CRISPR/Cas9 System [J].

Zaidi, Syed Shan-e-Ali ;

Mansoor, Shahid ;

Ali, Zahir ;

Tashkandi, Manal ;

Mahfouz, Magdy M. .

TRENDS IN PLANT SCIENCE, 2016, 21 (04) :279-281