Changes in the Two-Dimensional Electrophoresis Pattern of the Parkinson's Disease Related Protein DJ-1 in Human SH-SY5Y Neuroblastoma Cells After Dopamine Treatment

被引：8

作者：

Alberio, Tiziana ^{[1
]}

Colapinto, Monica ^{[1
]}

Natale, Massimo ^{[2
]}

Ravizza, Raffaella ^{[1
]}

Gariboldi, Marzia B. ^{[1
]}

Bucci, Enrico M. ^{[2
]}

Lopiano, Leonardo ^{[3
]}

Fasano, Mauro ^{[1
]}

机构：

[1] Univ Insubria, Dept Struct & Funct Biol, Ctr Neurosci, I-21052 Busto Arsizio, VA, Italy

[2] BioDigitalValley Srl, Pont St Martin, AO, Italy

[3] Univ Turin, Dept Neurosci, Turin, Italy

来源：

IUBMB LIFE | 2010年 / 62卷 / 09期

关键词：

dopamine; oxidative stress; DJ-1; Parkinson's disease; two-dimensional electrophoresis; ALPHA-SYNUCLEIN; OXIDATIVE DAMAGE; EXPRESSION; MUTATIONS; TOXICITY; STORAGE;

D O I：

10.1002/iub.371

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DJ-1 is a mitochondrial protein linked to Parkinson's disease. DJ-1 has been suggested to have several possible functions, although it has been mainly associated to oxidative stress defence. Changes in the two-dimensional electrophoresis pattern have been thoroughly described as a consequence of oxidative modification of the Cys106 residue. There is accumulating evidence supporting a specific role of DJ-1 in protecting dopaminergic neurons from dopamine itself. By exposing SH-SY5Y human neuroblastoma catecholaminergic cells to dopamine, we observed a specific increase in the most acidic forms in the DJ-1 two-dimensional electrophoresis pattern together with a significant decrease of the most basic spot. Unlike cells exposed to generic oxidative conditions, no additional shift was observed. The results are corroborated by a meta-analysis of the literature showing that in the absence of dopamine treatment the specific acidic form is underrepresented. (C) 2010 IUBMB IUBMB Life, 62(9): 688-692, 2010

引用

页码：688 / 692

页数：5

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