Regulation of IL-1-induced gingival collagenase gene expression by activator protein-1. (c-fos/c-jun)

被引:15
作者
Hamid, QA [1 ]
Reddy, PJ [1 ]
Tewari, M [1 ]
Uematsu, S [1 ]
Tuncay, OC [1 ]
Tewari, DS [1 ]
机构
[1] Temple Univ, Sch Dent, Dept Orthodont, Philadelphia, PA 19140 USA
关键词
c-fos; c-jun; collagenase; gingival fibroblasts; IL-1; periodontitis;
D O I
10.1006/cyto.2000.0676
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Matrix metalloproteinase-1 is probably involved in the progression of periodontal disease, The aim of this study was to investigate whether IL-1 beta stimulates the expression of the activator protein 1 (AP-1) transcription factor and, consequently, if the AP-1 transcription factor participates in the regulation of collagenase gene expression in human gingival fibroblast cells, In this study, we demonstrate that the concentration of the protein components of AP-1 transcription factor, c-Fos and c-Jun, is enhanced by IL-1 beta both at mRNA and protein levels, utilizing Northern blot analysis, electrophoretic mobility gel shift assay and Western blot analysis, The IL-1 beta stimulated the collagenase-CAT and AP-1-CAT activities in a dose dependent manner with respect to the amount of DNA used in transfections, Further, overexpression of c-Fos and c-Jun proteins revealed a dose-dependent transcriptional activation of the collagenase promoter. These findings, coupled with the existence of AP-1 consensus DNA binding sites on the collagenase gene promoter, show that regulation of collagenase gene expression by IL-1 beta involves the transcription factor AP-1 in gingival fibroblasts, (C) 2000 Academic Press.
引用
收藏
页码:1609 / 1619
页数:11
相关论文
共 39 条
[1]   INTERLEUKIN-1 STIMULATES ITS OWN RECEPTOR EXPRESSION ON HUMAN-FIBROBLASTS THROUGH THE ENDOGENOUS PRODUCTION OF PROSTAGLANDIN(S) [J].
AKAHOSHI, T ;
OPPENHEIM, JJ ;
MATSUSHIMA, K .
JOURNAL OF CLINICAL INVESTIGATION, 1988, 82 (04) :1219-1224
[2]   COMPLEXITY OF THE EARLY GENETIC RESPONSE TO GROWTH-FACTORS IN MOUSE FIBROBLASTS [J].
ALMENDRAL, JM ;
SOMMER, D ;
MACDONALDBRAVO, H ;
BURCKHARDT, J ;
PERERA, J ;
BRAVO, R .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (05) :2140-2148
[3]   THE ROLE OF JUN, FOS AND THE AP-1 COMPLEX IN CELL-PROLIFERATION AND TRANSFORMATION [J].
ANGEL, P ;
KARIN, M .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1072 (2-3) :129-157
[4]   PHORBOL ESTER INDUCIBLE GENES CONTAIN A COMMON CIS ELEMENT RECOGNIZED BY A TPA-MODULATED TRANS-ACTING FACTOR [J].
ANGEL, P ;
IMAGAWA, M ;
CHIU, R ;
STEIN, B ;
IMBRA, RJ ;
RAHMSDORF, HJ ;
JONAT, C ;
HERRLICH, P ;
KARIN, M .
CELL, 1987, 49 (06) :729-739
[5]  
AUSBEL FM, 1996, CURRENT PROTOCOLS MO
[6]   The AP-1 site and MMP gene regulation: What is all the fuss about? [J].
Benbow, U ;
Brinckerhoff, CE .
MATRIX BIOLOGY, 1997, 15 (8-9) :519-526
[7]   ROLE OF MATRIX METALLOPROTEINASES IN HUMAN PERIODONTAL-DISEASES [J].
BIRKEDALHANSEN, H .
JOURNAL OF PERIODONTOLOGY, 1993, 64 (05) :474-484
[8]   OVEREXPRESSION OF C-JUN, JUNB, OR JUND AFFECTS CELL-GROWTH DIFFERENTLY [J].
CASTELLAZZI, M ;
SPYROU, G ;
LAVISTA, N ;
DANGY, JP ;
PIU, F ;
YANIV, M ;
BRUN, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (20) :8890-8894
[9]   INCREASED THYMOCYTE-ACTIVATING FACTOR IN HUMAN GINGIVAL FLUID DURING GINGIVAL INFLAMMATION [J].
CHARON, JA ;
LUGER, TA ;
MERGENHAGEN, SE ;
OPPENHEIM, JJ .
INFECTION AND IMMUNITY, 1982, 38 (03) :1190-1195
[10]   Abrogation of interleukin-3 dependence of myeloid cells by the v-src oncogene requires SH2 and SH3 domains which specify activation of STATs [J].
Chaturvedi, P ;
Sharma, S ;
Reddy, EP .
MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (06) :3295-3304