Synthesis of hydrophilic boronate affinity monolithic capillary for specific capture of glycoproteins by capillary liquid chromatography

被引：106

作者：

Ren, Lianbing ^{[1
]}

Liu, Yunchun ^{[1
]}

Dong, Mingming ^{[1
]}

Liu, Zhen ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 47期

基金：

中国国家自然科学基金;

关键词：

Boronate affinity; Capillary liquid chromatography; Capture; Glycoprotein; Monolithic column; SHOTGUN PROTEOME ANALYSIS; MALDI-TOF/TOF-MS; MASS-SPECTROMETRY; SEPARATION; COLUMNS; PARTICLES; PROTEINS; POLYMERIZATION; MICROREACTOR; MS/MS;

D O I：

10.1016/j.chroma.2009.10.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Boronate affinity chromatography is an important tool for specific isolation of cis-diol-containing compounds such as glycoproteins, RNA and carbohydrates. Boronate functionalized monolithic capillaries have been recently developed for specific capture of cis-diol-containing small biomolecules, but the apparent hydrophobicity of the columns prevents them from specific capture of glycoproteins. In this paper, a hydrophilic boronate affinity monolithic capillary was prepared by in situ free radical polymerization, using 4-vinylphenylboronic acid (VPBA) and N, N'-methylenebisacrylamide (MBAA) as functional monomer and cross-linker, respectively. The prepared poly(VPBA-co-MBAA) monolithic capillary exhibited uniform open channel network and high density of accessible boronic acid. Due to the utilization of hydrophilic cross-linker, the prepared column was hydrophilic, allowing for specific capture of glycoproteins. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：8421 / 8425

页数：5

共 28 条

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