Neutrophil Migration into the Infected Uroepithelium Is Regulated by the Crosstalk between Resident and Helper Macrophages

被引：19

作者：

Zec, Kristina ^{[1
]}

Volke, Julia ^{[1
]}

Vijitha, Nirojah ^{[1
]}

Thiebes, Stephanie ^{[1
]}

Gunzer, Matthias ^{[1
]}

Kurts, Christian ^{[2
]}

Engel, Daniel Robert ^{[1
]}

机构：

[1] Univ Duisburg Essen, Univ Hosp Essen, Dept Immunodynam, Inst Expt Immunol & Imaging, D-45141 Essen, Germany

[2] Univ Hosp Bonn, Inst Expt Immunol, D-53127 Bonn, Germany

来源：

PATHOGENS | 2016年 / 5卷 / 01期

关键词：

uropathogenic E. coli (UPEC); Ly6C(+) and Ly6C(-) macrophages; transepithelial migration; neutrophils; crosstalk; BONE-MARROW; IN-VITRO; RECEPTOR; ACTIVATION; EXPRESSION; CELLS; PYELONEPHRITIS; INTERLEUKIN-8; MONOCYTES; IMMUNITY;

D O I：

10.3390/pathogens5010015

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The antibacterial defense against infections depends on the cooperation between distinct phagocytes of the innate immune system, namely macrophages and neutrophils. However, the mechanisms driving this cooperation are incompletely understood. In this study we describe the crosstalk between Ly6C(+) and Ly6C(-) macrophage-subtypes and neutrophils in the context of urinary tract infection (UTI) with uropathogenic E. coli (UPEC). Ly6C(-) macrophages acted as tissue resident sentinels and attracted circulating phagocytes by chemokines. Ly6C(+) macrophages produced tumor necrosis factor (TNF) that licensed Ly6C(-) macrophages to release preformed CXCL2, which in turn caused matrix metalloproteinases (MMP-9) secretion by neutrophils to enable transepithelial migration.

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相关论文

共 38 条

[1] The proinflammatory CD14+CD16+DR++ monocytes are a major source of TNF [J].

Belge, KU ;

Dayyani, F ;

Horelt, A ;

Siedlar, M ;

Frankenberger, M ;

Frankenberger, B ;

Espevik, T ;

Ziegler-Heitbrock, L .

JOURNAL OF IMMUNOLOGY, 2002, 168 (07) :3536-3542

[2] Macrophage polarization in bacterial infections [J].

Benoit, Marie ;

Desnues, Benoit ;

Mege, Jean-Louis .

JOURNAL OF IMMUNOLOGY, 2008, 181 (06) :3733-3739

[3] CCL5-enhanced human immature dendritic cell migration through the basement membrane in vitro depends on matrix metalloproteinase-9 [J].

Chabot, Valerie ;

Reverdiau, Pascale ;

Iochmann, Sophie ;

Rico, Angelique ;

Senecal, Delphine ;

Goupille, Caroline ;

Sizaret, Pierre-Yves ;

Sensebe, Luc .

JOURNAL OF LEUKOCYTE BIOLOGY, 2006, 79 (04) :767-778

[4] Regulation of matrix metalloproteinase-9 release from IL-8-stimulated human neutrophils [J].

Chakrabarti, S ;

Patel, KD .

JOURNAL OF LEUKOCYTE BIOLOGY, 2005, 78 (01) :279-288

[5] Neutrophil transepithelial migration and epithelial barrier function in IBD - Potential targets for inhibiting neutrophil trafficking [J].

Chin, Alex C. ;

Parkos, Charles A. .

INFLAMMATORY BOWEL DISEASE: GENETICS, BARRIER FUNCTION, IMMUNOLOGIC MECHANISMS, AND MICROBIAL PATHWAYS, 2006, 1072 :276-287

[6] CCR2 mediates homeostatic and inflammatory release of Gr1high monocytes from the bone marrow, but is dispensable for bladder infiltration in bacterial urinary tract infection [J].

Engel, Daniel R. ;

Maurer, Juliane ;

Tittel, Andre P. ;

Weisheit, Christina ;

Cavlar, Taner ;

Schumak, Beatrix ;

Limmer, Andreas ;

van Rooijen, Nico ;

Trautwein, Christian ;

Tacke, Frank ;

Kurts, Christian .

JOURNAL OF IMMUNOLOGY, 2008, 181 (08) :5579-5586

[7] TUMOR-NECROSIS-FACTOR-ALPHA IS REQUIRED IN THE PROTECTIVE IMMUNE-RESPONSE AGAINST MYCOBACTERIUM-TUBERCULOSIS IN MICE [J].

FLYNN, JL ;

GOLDSTEIN, MM ;

CHAN, J ;

TRIEBOLD, KJ ;

PFEFFER, K ;

LOWENSTEIN, CJ ;

SCHREIBER, R ;

MAK, TW ;

BLOOM, BR .

IMMUNITY, 1995, 2 (06) :561-572

[8]

Foxman B, 2002, AM J MED, V113, p5S

[9] Interleukin 8 receptor deficiency confers susceptibility to acute experimental pyelonephritis and may have a human counterpart [J].

Frendéus, B ;

Godaly, G ;

Hang, L ;

Karpman, D ;

Lundstedt, AC ;

Svanborg, C .

JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 192 (06) :881-890

[10] Fate Mapping Analysis Reveals That Adult Microglia Derive from Primitive Macrophages [J].

Ginhoux, Florent ;

Greter, Melanie ;

Leboeuf, Marylene ;

Nandi, Sayan ;

See, Peter ;

Gokhan, Solen ;

Mehler, Mark F. ;

Conway, Simon J. ;

Ng, Lai Guan ;

Stanley, E. Richard ;

Samokhvalov, Igor M. ;

Merad, Miriam .

SCIENCE, 2010, 330 (6005) :841-845

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