Molecular Cloning and Characterization of a Subtilisin-Like Serine Protease Gene (Pr1) from the Medicinal Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycetes)

被引：1

作者：

Shi, Ping ^{[1
]}

Lu, Zenghui ^{[1
]}

He, Yuanchuan ^{[1
]}

Chen, Shijiang ^{[1
]}

Yan, Jun ^{[2
]}

Li, Junhong ^{[2
]}

Zhang, Xiaobing ^{[2
]}

机构：

[1] Chongqing Acad Chinese Mat Med, Inst Chinese Caterpillar Fungus, Nanshan Rd, Chongqing 400065, Peoples R China

[2] Chongqing Taiji Ind Grp Co Ltd, Chongqing, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS | 2015年 / 17卷 / 11期

关键词：

medicinal mushrooms and fungi; subtilisin-like serine protease (Pr1); Ophiocordyceps sinensis; cloning; characterization; BEAUVERIA-BASSIANA; EXPRESSION; FUNGUS;

D O I：

10.1615/IntJMedMushrooms.v17.i11.90

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the Chinese caterpillar mushroom Ophiocordyceps sinensis, a subtislin-like serine protease (Pr1) is one of the most important enzymes for its infection activity against insect cuticles. The Pr1 gene was isolated from the valuable Chinese medicinal fungus O. sinensis using rapid amplification of 5' and 3' complementary DNA ends. The 2079-bp full-length complementary DNA sequence containing the 1605-bp predicted open reading frame of the Pr1 gene was obtained (GenBank accession no. KF836756). The open reading frame encodes a protein comprising 534 amino acids. Protein sequence multiple alignment analysis revealed high homology with 16 other subtilisin serine proteases and exhibited the highly conserved catalytic domain (D195, H227, and S393). We also constructed a phylogenetic tree in this study. Further molecular studies are needed to elucidate the mechanisms of fungal infection.

引用

页码：1087 / 1093

页数：7

共 24 条

[1] Genetic polymorphisms in three subtilisin-like protease isoforms (Pr1A, Pr1B, and Pr1C) from Metarhizium strains [J].

Bidochka, MJ ;

Melzer, MJ .

CANADIAN JOURNAL OF MICROBIOLOGY, 2000, 46 (12) :1138-1144

[2] Investigations on a hyper-proteolytic mutant of Beauveria bassiana: broad substrate specificity and high biotechnological potential of a serine protease [J].

Borgi, Ines ;

Gargouri, Ali .

FEMS MICROBIOLOGY LETTERS, 2014, 351 (01) :23-31

[3] Serine protease identification (in vitro) and molecular structure predictions (in silico) from a phytopathogenic fungus, Alternaria solani [J].

Chandrasekaran, Murugesan ;

Chandrasekar, Raman ;

Sa, Tongmin ;

Sathiyabama, Muthukrishnan .

JOURNAL OF BASIC MICROBIOLOGY, 2014, 54 :S210-S218

[4]

Charnley A.K., 1991, P267

[5]

Chu H., 2004, FAUNA SINICA INSECTA, V38, p[1, 254, 274]

[6] New insights into the mechanisms of fungal pathogenesis in insects [J].

Clarkson, JM ;

Charnley, AK .

TRENDS IN MICROBIOLOGY, 1996, 4 (05) :197-203

[7] Cloning and phylogenetic analysis of the chitinase gene from the facultative pathogen Paecilomyces lilacinus [J].

Dong, L. Q. ;

Yang, J. K. ;

Zhang, K. Q. .

JOURNAL OF APPLIED MICROBIOLOGY, 2007, 103 (06) :2476-2488

[8]

Feng J, 2010, MOL PLANT PATHOL, V11, P503, DOI [10.1111/j.1364-3703.2010.00623.x, 10.1111/J.1364-3703.2010.00623.X]

[9] Proteomic response of the biological control fungus Trichoderma atroviride to growth on the cell walls of Rhizoctonia solani [J].

Grinyer, J ;

Hunt, S ;

McKay, M ;

Herbert, BR ;

Nevalainen, H .

CURRENT GENETICS, 2005, 47 (06) :381-388

[10] Genome survey uncovers the secrets of sex and lifestyle in caterpillar fungus [J].

Hu Xiao ;

Zhang YongJie ;

Xiao GuoHua ;

Zheng Peng ;

Xia YongLiang ;

Zhang XingYu ;

St Leger, Raymond J. ;

Liu XingZhong ;

Wang ChengShu .

CHINESE SCIENCE BULLETIN, 2013, 58 (23) :2846-2854

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