Plasmodium falciparum:: Detection of a novel asparagine-rich protein on the surface of sporozoite

Zhang, K., Fujioka, H., Aikawa, M., and Kumar, N. 1999. Plasamodium falciparum: Detection of a novel asparagine-rich protein on the surface of sporozoite. Experimental Parasitology 93, 1-6. We had previously cloned and characterized a gene for a novel asparagine-rich protein from P. falciparum (PfARP). a target of natural human immune response. The antibodies to PfARP were localized to the surface of parasitized red blood cells and reacted with intracellular components in all erythrocytic asexual and sexual stages of the parasite. We here describe reactivity of antibodies against this novel PfARP on the surface of mosquito stage sporozoite of P. falciparum by indirect immunofluorescence assay and immunoelectron microscopy, the latter revealing a highly periodic punctate pattern of distribution of PfARP on the surface of sporozoite. These results suggest a possibility that PfARP might represent yet another sporozoite surface protein. (C) 1999 Academic Press.