TAF-ChIP: an ultra-low input approach for genome-wide chromatin immunoprecipitation assay

被引：12

作者：

Akhtar, Junaid ^{[1
]}

More, Piyush ^{[2
]}

Albrecht, Steffen ^{[5
]}

Marini, Federico ^{[3
,4
]}

Kaiser, Waldemar ^{[1
]}

Kulkarni, Apurva ^{[1
,6
]}

Wojnowski, Leszek ^{[2
]}

Fontaine, Jean-Fred ^{[5
]}

Andrade-Navarro, Miguel A. ^{[5
]}

Silies, Marion ^{[1
]}

Berger, Christian ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Inst Dev Biol & Neurobiol, Mainz, Germany

[2] Johannes Gutenberg Univ Mainz, Univ Med Ctr, Dept Pharmacol, Mainz, Germany

[3] Ctr Thrombosis & Hemostasis Mainz, Mainz, Germany

[4] Inst Med Biostat Epidemiol & Informat, Mainz, Germany

[5] Johannes Gutenberg Univ Mainz, Fac Biol, Mainz, Germany

[6] IISER Pune, Pune, Maharashtra, India

来源：

LIFE SCIENCE ALLIANCE | 2019年 / 2卷 / 04期

关键词：

TRANSCRIPTION FACTOR-BINDING; SEQ; PROTEINS; REVEALS; ELEMENTS;

D O I：

10.26508/lsa.201900318

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is a powerful technique to study transcriptional regulation. However, the requirement of millions of cells to generate results with high signal-to-noise ratio precludes it in the study of small cell populations. Here, we present a tagmentation-assisted fragmentation ChIP (TAF-ChIP) and sequencing method to generate high-quality histone profiles from low cell numbers. The data obtained from the TAF-ChIP approach are amenable to standard tools for ChIP-Seq analysis, owing to its high signal-to-noise ratio. The epigenetic profiles from TAF-ChIP approach showed high agreement with conventional ChIP-Seq datasets, thereby underlining the utility of this approach.

引用

页数：12

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