Rapid and Sensitive Method for Quantitative Determination of Lopinavir and Ritonavir in Human Plasma by Liquid Chromatography-Tandem Mass Specrtometry

被引：17

作者：

Temghare, G. A. ^{[1
]}

Shetye, S. S. ^{[1
]}

Joshi, S. S. ^{[1
]}

机构：

[1] KJ Somaiya Coll Sci & Commerce, Bombay 400077, Maharashtra, India

来源：

E-JOURNAL OF CHEMISTRY | 2009年 / 6卷 / 01期

关键词：

Human immunodeficiency virus (HIV)-1; Lopinavir; Ritonavir; LC-MS-MS; EFAVIRENZ;

D O I：

10.1155/2009/709478

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A rapid and sensitive liquid chromatography-mass spectrometric (LC-MS-MS) method for the simultaneous determination of lopinavir and ritonavir in human plasma using abacavir as internal standard has been developed and validated. Sample preparation of plasma involved solid phase extraction. Detection was performed using an Applied Biosystems Sciex API 2000 Mass spectrometer. The assay of lopinavir and ritonavir was linear over the range of 50 ng mL(-1) to 20000 ng mL(-1) and 20 ng mL(-1) to 3000 ng mL(-1) respectively with a precision of < 15% and accuracy in the range of 85-115%. The limit of quantification in plasma for lopinavir and ritonavir was 50 ng mL(-1) and 20 ng mL(-1) respectively. The described method has the advantage of being rapid and easy and it could be applied in therapeutic monitoring of these drugs in human plasma.

引用

页码：223 / 230

页数：8

共 6 条

[1] *ABB LAB, 2000, PROD INF
[2] Efavirenz
Adkins, JC
Noble, S
[J]. DRUGS, 1998, 56 (06) : 1055 - 1064
[3] Corbett AH, 2002, ANN PHARMACOTHER, V36, P1193
[4] Determination of lopinavir and nevirapine by high-performance liquid chromatography after solid-phase extraction:: application for the assessment of their transplacental passage at delivery
Marzolini, C
Béguin, A
Telenti, A
Schreyer, A
Buclin, T
Biollaz, J
Decosterd, LA
[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 774 (02): : 127 - 140
[5] Simultaneous determination of indinavir, ritonavir and lopinavir (ABT 378) in human plasma by high-performance liquid chromatography
Ray, J
Pang, E
Carey, D
[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 775 (02): : 225 - 230
[6] Usami Y, 2003, CHEM PHARM BULL, V51, P715

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