Dual role of immunomodulation by crude polysaccharide from okra against carcinogenic liver injury in mice

被引:16
作者
Hayaza, Suhailah [1 ,2 ]
Wahyuningsih, Sri Puji Astuti [2 ]
Susilo, Raden Joko Kuncoroningrat [1 ,2 ]
Husen, Saikhu Akhmad [2 ]
Winarni, Dwi [2 ]
Doong, Ruey-an [2 ,3 ]
Darmanto, Win [1 ,2 ]
机构
[1] Univ Airlangga, Fac Sci & Technol, Lab Anim Physiol, Kampus C, Surabaya 60115, Indonesia
[2] Univ Airlangga, Fac Sci & Technol, Dept Biol, Kampus C, Surabaya 60115, Indonesia
[3] Natl Tsing Hua Univ, Inst Analyt & Environm Sci, Sec 2 Kuang Fu Rd, Hsinchu 30013, Taiwan
关键词
Okra; Raw polysaccharide extract; Diethylnitrosamine; Immunomodulator; Liver injury; CYCLOPHOSPHAMIDE-INDUCED IMMUNOSUPPRESSION; REGULATORY T-CELLS; ALPHA-FETOPROTEIN; TUMOR;
D O I
10.1016/j.heliyon.2021.e06183
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Polysaccharides have long been recognized as the anticancer agent with low toxicity and slight side effects. Okra (Abelmoschus esculentus L.), a flowering plant from the Malvaceae family that is found in tropical, subtropical, and warm temperate regions around the world. Hence, no in vivo studies have addressed the anticancer and immunomodulatory potential of polysaccharides from okra pods grown in Indonesia. This study aims to investigate the effect of okra raw polysaccharide extract (ORPE) to immune cells and cytokines of mice with hepatocarcinogenic conditions induced by diethylnitrosamine (DEN). Thirty-six male mice (BALB/C, 3-4 months old) were divided into six groups: the normal control group (CN), negative control (C-), positive control giving doxorubicin (C+), and three groups of ORPE treatment given the dose of 50 (P1), 100 (P2) and 200 (P3) mg/kg body weight. The administration of ORPE directly suppressed the regulatory T cells accumulations, suppressed macrophages activations, and balanced the number of effector T cells. However, it promoted CD8+ T cell activation at a low dose and increased interleukin-2 levels at all doses. These results suggest that ORPE has unique dual-functions as immunosuppressant and immunostimulant which can be a foundation for the application of the ORPE in the nutraceutical and pharmaceutical industries.
引用
收藏
页数:7
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