Practical Method for the Detection of Tetracyclines in Honey by HPLC and Derivative UV-Vis Spectra

被引:16
作者
Hakuta, Tadao [2 ]
Shinzawa, Hideyuki [3 ]
Ozaki, Yukihiro [1 ]
机构
[1] Kwansei Gakuin Univ, Dept Chem, Sch Sci & Technol, Nishinomiya, Hyogo 6691337, Japan
[2] Ibaraki Prefectural Inst Publ Hlth, Mito, Ibaraki 3100852, Japan
[3] AIST, Res Inst Instrumentat Frontier, Nagoya, Aichi 4638560, Japan
关键词
PERFORMANCE LIQUID-CHROMATOGRAPHY; RESIDUAL TETRACYCLINES; CHEMICAL-ANALYSIS; ANTIBIOTICS; OXYTETRACYCLINE; IMPROVEMENT; RESISTANCE; ANIMALS; HUMANS; FOODS;
D O I
10.2116/analsci.25.1149
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A technique to detect residual tetracyclines (TCs) in honey by HPLC and derivative UV-Vis spectra is examined in this study. Chromatograms of honey products often experience overlapped peaks and sloping background due to similar retention properties of components or to the presence of impurities. The derivatives provide resolution of overlapped peaks in an UV-Vis spectrum and eventually bring chromatograms that show pure elution behavior of TCs. Calibration models for oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) are built out of the derivative spectra of standard solutions of honey and their prediction abilities are examined. The results show that the calibration models for OTC, TC and CTC can yield sufficient levels for the determination. The technique reported here may be effectively used for quantitative determination of TCs.
引用
收藏
页码:1149 / 1153
页数:5
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