Determination of riboflavin by high-performance liquid chromatography with riboflavin-depleted urine as calibration and control matrix

被引:20
作者
Chen, M [1 ]
Andrenyak, DM [1 ]
Moody, DE [1 ]
Foltz, RL [1 ]
机构
[1] Univ Utah, Ctr Human Toxicol, Dept Pharmacol & Toxicol, Salt Lake City, UT 84112 USA
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 820卷 / 01期
关键词
riboflavin; urine; HPLC; stability;
D O I
10.1016/j.jchromb.2005.03.018
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple method, exposure to natural-light, was developed to remove riboflavin from urine to enhance its use as the biological matrix for the preparation of calibration and control samples. Riboflavin-depleted urine containing less than 1 ng/ml of riboflavin was used to validate a high-performance liquid chromatography with fluorescence detection method for the determination of urinary riboflavin. The linearity of the assay (r(2) = 0.999) was acceptable over the range of 10-5000 ng/ml. The intra-assay and inter-assay CVs were 3.3% and 9%, respectively. Subsequent stability studies found that urine riboflavin was stable for up to 6 months at 4 or -20 degrees C. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:147 / 150
页数:4
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