Microfluidic paper-based analytical device by using Pt nanoparticles as highly active peroxidase mimic for simultaneous detection of glucose and uric acid with use of a smartphone

被引：51

作者：

Zheng, Jie ^{[1
]}

Zhu, Min ^{[2
]}

Kong, Jiao ^{[1
]}

Li, Zimu ^{[1
]}

Jiang, Jianming ^{[1
]}

Xi, Yachao ^{[1
]}

Li, Fang ^{[1
]}

机构：

[1] Hefei Univ Technol, Sch Chem & Chem Engn, Anhui Prov Key Lab Adv Catalyt Mat & React Engn, Hefei 230009, Anhui, Peoples R China

[2] PLA Army Acad Artillery & Air Def, Hefei 230031, Anhui, Peoples R China

来源：

TALANTA | 2022年 / 237卷

基金：

中国国家自然科学基金;

关键词：

mu PAD; Pt NPs; Glucose; Uric acid; Smartphone; COLORIMETRIC ASSAY;

D O I：

10.1016/j.talanta.2021.122954

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Herein, a simple microfluidic paper-based analytical device (mu PAD) by using platinum nanoparticles (Pt NPs) as highly active peroxidase mimic for simultaneous determination of glucose and uric acid was fabricated. The mu PAD consisted of one sample transportation layer, four paper-based detection chips, and two layers of hydrophobic polyethylene terephthalate (PET) films. The four detection chips were immobilized with various chromogenic reagents, Pt NPs, and specific oxidase (glucose oxidase or uricase). H2O2 generated by specific enzymatic reactions could oxidize co-immobilized chromogenic reagents to produce colored products by using Pt NPs as efficient catalyst. The multi-layered structure of mu PAD could effectively improve the color uniformity and color intensity. Total color intensity from each two detection chips modified with distinct chromogenic reagents were used for quantitative analysis of glucose and uric acid, respectively, resulting in significantly improved sensitivity. The linear range for glucose and uric acid detection was 0.01-5.0 mM and 0.01-2.5 mM, respectively. Satisfied results were obtained for glucose and uric acid detection in real serum samples. An easy-to-use smartphone APP was developed for convenient and intelligent detection. The developed mu PAD integrated with smartphone as detector holds great applicability for simple and portable on-site analysis.

引用

页数：9

共 36 条

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