Proximate analysis, Antioxidant Property and Cytotoxicity Assessment of Pseuderanthemum reticulaturn Leaves

The bioactive constituents derived from plants attract the attention of researchers due to their potential applications in the medicinal field. In this regard, the proximate analysis and the cytotoxicity study of the plant materials play an important role in the phytochemical research. In the present work, estimation of total ash, moisture content, fiber content, crude protein, and carbohydrate were carried out under proximate analysis and the antioxidant activity of the anthocyanin present in the plant material was evaluated by DPPH(2,2-diphenyl-1-picrylhydrazyl) method. The separation of anthocyanin pigment from the plant material was done by paper chromatography (PC) technique and they are characterized by UV-spectrum, chemical test and the R, values obtained from paper chromatography. This study also investigated the in vitrocytotoxicity of Pseuderanthemum reticulatum leaves by means of MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)) assay using PBMC (Peripheral Blood Mononuclear Cell). The results of the proximate analysis showed that the plant material contains 7.6% of moisture content, 16.6% of total ash, 5.6% of crude protein, 23.0% of crude fiber, 3.82% of crude fat and 23.64% of carbohydrate. The free radical scavenging ability of the separated anthocyanin was found to be 72.58% at 10 mu g/mL. The cytotoxicity investigation showed that the aqueous extract possess the IC50 value of 161.5 mu g/mL. The high percentage of radical scavenging activity and low toxicity of the plant suggest that it can be extensively used for the investigation of the bioactive constituents and its applications.