Transparent magnetic photoresists for bioanalytical applications

被引：24

作者：

Gach, Philip C. ^{[1
]}

Sims, Christopher E. ^{[1
]}

Allbritton, Nancy L. ^{[1
,2
,3
]}

机构：

[1] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Dept Biomed Engn, Chapel Hill, NC 27599 USA

[3] N Carolina State Univ, Raleigh, NC 27695 USA

来源：

BIOMATERIALS | 2010年 / 31卷 / 33期

基金：

美国国家卫生研究院;

关键词：

Magnetism; Photolithography; Biocomaptibility; Micropatterning; MTT assay; Cell adhesion; IRON-OXIDE NANOPARTICLES; BIOCOMPATIBILITY; EXPANSION; CELLS;

D O I：

10.1016/j.biomaterials.2010.07.087

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Microfabricated devices possessing magnetic properties are of great utility in bioanalytical microdevices due to their controlled manipulation with external magnets. Current methods for creating magnetic microdevices yield a low-transparency material preventing light microscopy-based inspection of biological specimens on the structures. Uniformly transparent magnetic photoresists were developed for microdevices that require high transparency as well as consistent magnetism across the structure. Colloidal formation of 10 nm maghemite particles was minimized during addition to the negative photoresists SU-8 and 1002F through organic capping of the nanoparticles and utilization of solvent-based dispersion techniques. Photoresists with maghemite concentrations of 0.01-1% had a high transparency due to the even dispersal of maghemite nanoparticles within the polymer as observed with transmission electron microscopy (TEM). These magnetic photoresists were used to fabricate microstructures with aspect ratios up to 4:1 and a resolution of 3 pm. Various cell lines showed excellent adhesion and viability on the magnetic photoresists. An inspection of cells cultured on the magnetic photoresists with TEM showed cellular uptake of magnetic nanoparticles leeched from the photoresists. Cellular contamination by magnetic nanoparticles was eliminated by capping the magnetic photoresist surface with native 1002F photoresist or by removing the top layer of the magnetic photoresist through surface roughening. The utility of these magnetic photoresists was demonstrated by sorting single cells (HeLa, RBL and 3T3 cells) cultured on arrays of releasable magnetic micropallets. 100% of magnetic micropallets with attached cells were collected following release from the array. 85-92% of the collected cells expanded into colonies. The polymeric magnetic materials should find wide use in the fabrication of microstructures for bioanalytical technologies. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：8810 / 8817

页数：8

共 41 条

[11] Superparamagnetic polymer nanocomposites with uniform Fe3O4 nanoparticle dispersions [J].

Gas, J ;

Poddar, P ;

Almand, J ;

Srinath, S ;

Srikanth, H .

ADVANCED FUNCTIONAL MATERIALS, 2006, 16 (01) :71-75

[12]

GELORME JD, 1989, Patent No. 4882245

[13] Recent advances on surface engineering of magnetic iron oxide nanoparticles and their biomedical applications [J].

Gupta, Ajay Kumar ;

Naregalkar, Rohan R. ;

Vaidya, Vikas Deep ;

Gupta, Mona .

NANOMEDICINE, 2007, 2 (01) :23-39

[14] Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications [J].

Gupta, AK ;

Gupta, M .

BIOMATERIALS, 2005, 26 (18) :3995-4021

[15] Cell Uptake and in Vitro Toxicity of Magnetic Nanoparticles Suitable for Drug Delivery [J].

Hafelli, Urs O. ;

Riffle, Judy S. ;

Harris-Shekhawat, Linda ;

Carmichael-Baranauskas, Anita ;

Mark, Framin ;

Dailey, James P. ;

Bardenstein, David .

MOLECULAR PHARMACEUTICS, 2009, 6 (05) :1417-1428

[16] Large throw magnetic microactuator [J].

Hartley, A. C. ;

Miles, R. E. ;

Corda, J. ;

Dimitrakopoulos, N. .

MECHATRONICS, 2008, 18 (09) :459-465

[17] Synthesis of highly transparent lithium ferrite nanoparticle/polymer hybrid self-standing films exhibiting Faraday rotation in the visible region [J].

Hayashi, Koichiro ;

Fujilkawa, Rintaro ;

Sakamoto, Wataru ;

Inoue, Mitsuteru ;

Yogo, Toshinobu .

JOURNAL OF PHYSICAL CHEMISTRY C, 2008, 112 (37) :14255-14261

[18] Buried microchannels in photopolymer for delivering of solutions to neurons in a network [J].

Heuschkel, MO ;

Guerin, L ;

Buisson, B ;

Bertrand, D ;

Renaud, P .

SENSORS AND ACTUATORS B-CHEMICAL, 1998, 48 (1-3) :356-361

[19] Magnetic Nanocrystals Modified Epoxy Photoresist for Microfabrication of AFM probes [J].

Ingrosso, C. ;

Martin, C. ;

Llobera, A. ;

Murano, F. Perez ;

Innocenti, C. ;

Sangregorio, C. ;

Voigt, A. ;

Gruetzner, G. ;

Brugger, J. ;

Striccoli, M. ;

Agostiano, A. ;

Curri, M. L. .

PROCEEDINGS OF THE EUROSENSORS XXIII CONFERENCE, 2009, 1 (01) :580-+

[20] Biodistribution, clearance, and biocompatibility of iron oxide magnetic nanoparticles in rats [J].

Jain, Tapan K. ;

Reddy, Maram K. ;

Morales, Marco A. ;

Leslie-Pelecky, Diandra L. ;

Labhasetwar, Vinod .

MOLECULAR PHARMACEUTICS, 2008, 5 (02) :316-327

← 1 2 3 4 5 →