Different susceptibility to oxidation of proline and arginine residues of apolipoprotein B-100 among subspecies of low density lipoproteins

gamma -Glutamyl semialdehyde is a primary oxidation product of apolipoprotein (apo) B-100 proline (Pro) and arginine (Arg) side chain residues, By reduction gamma -glutamyl semialdehyde forms 5-hydroxy-2-aminovaleric acid (HAVA). Here me describe the application of sensitive and specific HAVA measurement to characterize the formation of gamma -glutamyl semialdehyde in several domains of apoB-100 in LDLI (S-f 7-12) and LDL2 (S-f 0-7) subfractions subjected to oxidative damage in the presence of iron in vitro. Results suggest that susceptibility of apoB-100 Pro and Arg residues toward oxygen radicals drastically changes along the lipoprotein metabolic cascade. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.