Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae

被引:43
作者
Bian, Chun [1 ,2 ]
Wang, Zhenyu [1 ]
Shi, John [1 ,3 ]
机构
[1] Harbin Inst Technol, Sch Chem & Chem Engn, Harbin 150001, Peoples R China
[2] Harbin Univ, Sch Food Engn, Harbin 150086, Peoples R China
[3] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada
来源
MOLECULES | 2020年 / 25卷 / 03期
基金
中国国家自然科学基金;
关键词
corresponding surface optimization; ultrasound assisted extraction; chemical composition; anticoagulation in vitro; ULTRASOUND-ASSISTED EXTRACTION; DEGRADATION; ANTIOXIDANT; EAR;
D O I
10.3390/molecules25030710
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM). The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 degrees C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W). Furthermore, the acidic AAP (aAAP) was precipitated with cetyltrimethylammonium bromide (CTAB) from crude AAP (cAAP). aAAP was further purified using ion exchange chromatography with a DEAE Purose 6 Fast Flow column to obtain aAAP-1. Additionally, according to the HPLC analysis, the aAAP-1 was mainly composed of mannose, glucuronic acid, glucose, galactose, and xylose, with a molar ratio of 80.63:9.88:2.25:1:31.13. Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.
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页数:14
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