Water-Soluble Molecularly Imprinted Nanoparticles (MINPs) with Tailored, Functionalized, Modifiable Binding Pockets

Construction of receptors with binding sites of specific size, shape, and functional groups is important to both chemistry and biology. Covalent imprinting of a photo-cleavable template within surface-core doubly cross-linked micelles yielded carboxylic acid-containing hydrophobic pockets within the water-soluble molecularly imprinted nanoparticles. The functionalized binding pockets were characterized by their binding of amine-and acid-functionalized guests under different pH values. The nanoparticles, on average, contained one binding site per particle and displayed highly selective binding among structural analogues. The binding sites could be modified further by covalent chemistry to modulate their binding properties.