The E3 Ligase GmPUB21 Negatively Regulates Drought and Salinity Stress Response in Soybean

被引：21
作者：
Yang, Yunhua [1 ]
Karthikeyan, Adhimoolam [2 ]
Yin, Jinlong [1 ]
Jin, Tongtong [3 ]
Ren, Rui [1 ]
Fang, Fei [1 ]
Cai, Han [1 ]
Liu, Mengzhuo [1 ]
Wang, Dagang [1 ]
Li, Kai [1 ]
Zhi, Haijian [1 ]
机构：
[1] Nanjing Agr Univ, Key Lab Biol & Genet Improvement Soybean, Natl Key Lab Crop Genet & Germplasm Enhancement, Natl Ctr Soybean Improvement,Minist Agr, Nanjing 210095, Peoples R China
[2] Jeju Natl Univ, Subtrop Hort Res Inst, Jeju 63243, South Korea
[3] Henan Agr Univ, Coll Agron, Ctr Crop Genome Engn, Zhengzhou 450046, Peoples R China
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 13期
关键词：
abscisic acid; abiotic stress; GmPUB21; ubiquitination; stomata; UBIQUITIN LIGASE; TRANSCRIPTION FACTOR; SIGNAL-TRANSDUCTION; ABSCISIC-ACID; SALT STRESS; TOLERANCE; EXPRESSION; ROLES; GENE; UBIQUITYLATION;
D O I：
10.3390/ijms23136893
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
E3-ubiquitin ligases are known to confer abiotic stress responses in plants. In the present study, GmPUB21, a novel U-box E3-ubiquitin ligase-encoding gene, was isolated from soybean and functionally characterized. The expression of GmPUB21, which possesses E3-ubiquitin ligase activity, was found to be significantly up-regulated by drought, salinity, and ABA treatments. The fusion protein GmPUB21-GFP was localized in the cytoplasm, nucleus, and plasma membrane. Transgenic lines of the Nicotiana benthamiana over-expressing GmPUB21 showed more sensitive to osmotic, salinity stress and ABA in seed germination and inhibited mannitol/NaCl-mediated stomatal closure. Moreover, higher reactive oxygen species accumulation was observed in GmPUB21 overexpressing plants after drought and salinity treatment than in wild-type (WT) plants. Contrarily, silencing of GmPUB21 in soybean plants significantly enhanced the tolerance to drought and salinity stresses. Collectively, our results revealed that GmPUB21 negatively regulates the drought and salinity tolerance by increasing the stomatal density and aperture via the ABA signaling pathway. These findings improved our understanding of the role of GmPUB21 under drought and salinity stresses in soybean.
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页数：20
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