Host Response of Atlantic Salmon (Salmo salar) Re-Inoculated with Paramoeba perurans

被引：2

作者：

McCormack, Michelle ^{[1
]}

Talbot, Anita ^{[1
]}

Dillon, Eugene ^{[2
]}

O'Connor, Ian ^{[1
]}

MacCarthy, Eugene ^{[1
]}

机构：

[1] Galway Mayo Inst Technol, Marine & Freshwater Res Ctr, Dublin Rd, Galway H91 TRNW, Ireland

[2] Univ Coll Dublin, Conway Inst Biomol & Biomed Res, Dublin 4, Ireland

来源：

MICROORGANISMS | 2021年 / 9卷 / 05期

关键词：

amoebic gill disease; immune response; gill proteome; repeat inoculation; AMEBIC-GILL-DISEASE; IMMUNE GENE-EXPRESSION; NEOPARAMOEBA-PERURANS; FUNCTIONAL-CHARACTERIZATION; L; IDENTIFICATION; PROTEINS; CDNA; AGD; CYTOSCAPE;

D O I：

10.3390/microorganisms9050993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In aquaculture, recurrence rates of amoebic gill disease (AGD) caused by the ectoparasite Paramoeba perurans are high and no prophylactic strategies exist for disease prevention. In this study, Atlantic salmon (Salmo salar) were initially inoculated with P. perurans and following the development of amoebic gill disease were treated with freshwater immersion on day 21 and day 35 post inoculation. Fish were re-inoculated following a negative qPCR analysis for the presence of P. perurans. The gill host immune response was investigated at 7, 14, and 18 days post re-inoculation. Differential proteome expression of immune related proteins was assessed by comparison of each time point against naive controls. In the gill, some proteins of the innate immune system were expressed in response to gill re-colonization by P. perurans, while no features of adaptive immunity were found to be differentially expressed. Many of the proteins identified are novel in the context of AGD and their expression profiles suggest that their roles in the response to disease development and progression in single or multiple infections warrant further investigation.

引用

页数：15

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