Use of mailed urine specimens in diagnosing urothelial carcinoma by cytology and DNA image analysis

Objective To evaluate the usefulness of urine specimens collected via a mailer system and analyzed by cytology and DNA ploidy for the detection of urothelial carcinoma (UC). Study Design We retrospectively reviewed the diagnoses of 91 mailed urine specimens received from 72 patients, 67% of whom, had a history of UC. The specimens were fixed in, an equal volume of 50% ethanol solution before being mailed. The cytologic findings were interpreted in conjunction with DNA ploidy image analysis. We compared these initial diagnoses with those of follow-zip examinations, including biopsies, cystoscopic findings and urinary cytology/DNA ploidy analyses. In addition, to examine the quality of the mailed samples, 3 cytopathologists performed a blinded assessment of cytologic slides of 20 mailed and 17 fresh urinary samples for bacterial overgrowth, urothelial degeneration, and presence of proteinaceous material and crystals. Results Follow-up was available for 68 of the 91 mailed specimens. The sensitivity for detecting UC using mailed urine specimens that were analyzed by both cytology and DNA ploidy was 61 %, while specificity was 92 %. The levels of bacterial overgrowth and urothelial degeneration in the mailed specimens were not significantly greater than in the fresh specimens (p > 0.05). The levels of proteinaceous material and crystals were significantly higher in. the mailed specimens (p < 0.05).