Cross-priming amplification targeting the 18S rRNA gene for the rapid diagnosis of Babesia bovis infection

被引：5

作者：

Wang, Jinming ^{[1
]}

Gao, Shandian ^{[1
]}

Yang, Jifei ^{[1
]}

Liu, Junlong ^{[1
]}

Li, Youquan ^{[1
]}

Luo, Jianxun ^{[1
]}

Guan, Guiquan ^{[1
]}

Yin, Hong ^{[1
,2
]}

机构：

[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Key Lab Vet Parasitol Gansu Prov, State Key Lab Vet Etiol Biol, Xujiaping 1, Lanzhou 730046, Gansu, Peoples R China

[2] Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

来源：

TICKS AND TICK-BORNE DISEASES | 2021年 / 12卷 / 04期

基金：

国家重点研发计划;

关键词：

Cross-priming amplification; Diagnosis; Bovine babesiosis; Babesia bovis; CATTLE; PIROPLASMS; BIGEMINA; OVATA;

D O I：

10.1016/j.ttbdis.2021.101713

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Babesia bovis is a known causative agent of bovine babesiosis and is widely distributed across China. Rapid detection and accurate identification of B. bovis is essential for follow-up management and epidemiological investigations. In this study, a cross-priming amplification combined with vertical flow (CPA-VF) assay was developed. The detection limit of the CPA-VF assay targeting the 18S rRNA gene was 320 fg per reaction at 61 degrees C for 60 min. No cross-reactions were observed with other piroplasms infective to cattle. Furthermore, 36 blood samples from experimentally-infected animals were accurately assessed using the CPA-VF assay. The performance of the CPA-VF assay was compared with the results of conventional PCR for 219 blood samples from the field. Our results demonstrate that the CPA-VF assay is a practical and effective diagnostic tool for bovine babesiosis caused by B. bovis infection.

引用

页数：5