Towards a systematic validation of references in real-time RT-PCR

被引：193

作者：

Gutierrez, Laurent ^{[1
]}

Mauriat, Melanie ^{[1
]}

Pelloux, Jerome ^{[2
]}

Bellini, Catherine ^{[1
,3
]}

van Wuytswinkel, Olivier ^{[2
]}

机构：

[1] Swedish Univ Agr Sci, Umea Plant Sci Ctr, Dept Forest Genet & Plant Physiol, SE-90187 Umea, Sweden

[2] Univ Picardie Jules Verne, Fac Sci, EA3900 BioPl, F-80039 Amiens, France

[3] INRA, Biol Cellulaire Lab, F-78026 Versailles, France

来源：

PLANT CELL | 2008年 / 20卷 / 07期

关键词：

D O I：

10.1105/tpc.108.059774

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：1734 / 1735

页数：2

共 15 条

[1] Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets [J].

Andersen, CL ;

Jensen, JL ;

Orntoft, TF .

CANCER RESEARCH, 2004, 64 (15) :5245-5250

[2] Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis [J].

Czechowski, T ;

Stitt, M ;

Altmann, T ;

Udvardi, MK ;

Scheible, WR .

PLANT PHYSIOLOGY, 2005, 139 (01) :5-17

[3] Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors:: unprecedented sensitivity reveals novel root- and shoot-specific genes [J].

Czechowski, T ;

Bari, RP ;

Stitt, M ;

Scheible, WR ;

Udvardi, MK .

PLANT JOURNAL, 2004, 38 (02) :366-379

[4] The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization [J].

Dheda, K ;

Huggett, JF ;

Chang, JS ;

Kim, LU ;

Bustin, SA ;

Johnson, MA ;

Rook, GAW ;

Zumla, A .

ANALYTICAL BIOCHEMISTRY, 2005, 344 (01) :141-143

[5] Real-time PCR: what relevance to plant studies? [J].

Gachon, C ;

Mingam, A ;

Charrier, B .

JOURNAL OF EXPERIMENTAL BOTANY, 2004, 55 (402) :1445-1454

[6]

GUTIERREZ L, 2008, PLANT BIOTE IN PRESS

[7] Real-time RT-PCR normalisation; strategies and considerations [J].

Huggett, J ;

Dheda, K ;

Bustin, S ;

Zumla, A .

GENES AND IMMUNITY, 2005, 6 (04) :279-284

[8] Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress [J].

Nicot, N ;

Hausman, JF ;

Hoffmann, L ;

Evers, D .

JOURNAL OF EXPERIMENTAL BOTANY, 2005, 56 (421) :2907-2914

[9] Quantification of mRNA using real-time RT-PCR [J].

Nolan, Tania ;

Hands, Rebecca E. ;

Bustin, Stephen A. .

NATURE PROTOCOLS, 2006, 1 (03) :1559-1582

[10] Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR -: art. no. e36 [J].

Pfaffl, MW ;

Horgan, GW ;

Dempfle, L .

NUCLEIC ACIDS RESEARCH, 2002, 30 (09)

← 1 2 →