Physicochemical and antibacterial properties of fabricated ovalbumin-carvacrol gel nanoparticles

被引:0
|
作者
Rao, Sheng-qi [1 ,2 ,3 ]
Xu, Guang-wei [1 ]
Zeng, Hua-wei [4 ]
Zheng, Xiang-feng [1 ]
Hu, Qin [1 ]
Wang, Qi-Yan [5 ]
Yang, Zhen-quan [1 ,3 ]
Jiao, Xin-an [2 ,3 ]
机构
[1] Yangzhou Univ, Coll Food Sci & Engn, Yangzhou 225127, Jiangsu, Peoples R China
[2] Yangzhou Univ, Coll Biosci & Biotechnol, Postdoctoral Mobile Stn Biol, Yangzhou 225009, Jiangsu, Peoples R China
[3] Minist Agr China, Key Lab Prevent & Control Biol Hazard Factors Ani, Yangzhou 225001, Jiangsu, Peoples R China
[4] Huaibei Normal Univ, Coll Life Sci, Dept Bioengn, Huaibei 235000, Anhui, Peoples R China
[5] State Key Lab Nonfood Biomass & Enzyme Technol, Nanning 530226, Guangxi, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
HEAT-SET GELS; RHEOLOGICAL PROPERTIES; PROTEIN INTERACTIONS; MOLECULAR FORCES; PH; AGGREGATION; WHEY; COMPLEXES; DIGESTION; CHITOSAN;
D O I
10.1039/d0fo00755b
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The applications of carvacrol are limited due to its poor stability and water solubility, and high volatility; however, ovalbumin can be used to encapsulate hydrophobic molecules, improve their aqueous solubility, and reduce their volatility. In this study, we fabricated ovalbumin-carvacrol nanoparticles (OCG(n)s) under different pH (2, 5, 7, and 9) conditions using a gel embedding method and investigated their physicochemical and antibacterial properties. Rheological experiments revealed that theG ' of ovalbumin gels (OGs) prepared under different pH conditions were OG-2 > OG-7 > OG-9 > OG-5. Carvacrol addition reduced the tight structure of ovalbumin and carvacrol under pH 5 and 7 conditions, with hardness first decreasing and then increasing, but increasing under pH 2 and 9 conditions. Fluorescence and infrared spectroscopy indicated complex formation, with carvacrol increasing the average diameter of nanoparticles prepared at pH 2, 5, 7, and 9. Encapsulation reached 89.34 and 91.86% at pH 2 and 9, respectively; however, inhibition experiments revealed that the minimum inhibitory concentration of OCG(n)-2 against Gram-positiveBacillus cereusandSalmonella(0.08 and 0.16 mg mL(-1), respectively) was lower than that of OCG(n)-9 (both 0.28 mg mL(-1)). Moreover, OCG(n)-2 possessed a better dense gel structure and a higher stability, encapsulation rate, and antibacterial activity, suggesting that pH affects gel microstructure and thus the encapsulation efficiency and bacteriostatic properties of the prepared nanoparticles. These results contribute to our knowledge of the design and fabrication of polymeric nanoparticle delivery systems for bioactive compounds with beneficial properties.
引用
收藏
页码:5133 / 5141
页数:9
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