VIRULENCE FACTORS IN COAGULASE-NEGATIVE STAPHYLOCOCCI ISOLATED FROM COWS WITH SUBCLINICAL MASTITIS

被引：0

作者：

Malinowski, Edward ^{[1
]}

Klossowska, Anna ^{[1
]}

Zastempowska, Ewa ^{[1
]}

机构：

[1] Natl Inst Vet Res, Dept Pathophysiol Reprod & Mammary Gland, PL-85090 Bydgoszcz, Poland

来源：

BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY | 2011年 / 55卷 / 04期

关键词：

cow; mastitis; coagulase-negative staphylococci; virulence factors; FRAGMENT-LENGTH-POLYMORPHISM; BOVINE MASTITIS; BIOFILM FORMATION; MULTIPLEX PCR; AUREUS; IDENTIFICATION; PERSISTENCE; PREVALENCE; STRAINS; GENE;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The aim of this study was to investigate the presence of virulence genes in 46 coagulase-negative staphylococci (CNS) strains isolated from 46 udder quarters of 46 cows with subclinical mastitis. The isolates were examined phenotypically (cultural features, Gram stain, catalase, coagulase, API tests) and genotypically for: icaA, icaAB, icaD, atlE, bhp, agrA, sarA, mecA, and blaZ genes. These isolates had no nuc and coo genes, and were identified as S. warneri (11 strains), S. chromogenes (10), S. xylosus (seven), S. epidermidis (one), S. hyicus (one), and other Staphylococcus species (16). The single virulence genes or their combinations were detected in nine (19.6 %) of isolates. The following genes were found: blaZ (in four isolates), mecA (3), sarA (3), agrA (3), and atlE (three). None of the strains harboured the bhp, icaA, icaD, and icaAB genes. In conclusion, subclinical mastitis in cows can be caused by different species of CNS that harbour different virulence factors.

引用

页码：681 / 684

页数：4

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