Population genetic structure of the endangered freshwater crayfish Austropotamobius pallipes, assessed using RAPD markers

Random amplified polymorphic DNA (RAPD) analysis was performed to characterize the genetic diversity of Austropotamobius pallipes, a threatened freshwater crayfish native to Europe. Four decamer primers which generated six unambiguous polymorphic bands were used to analyse crayfish from 21 populations sampled in the major part of its range. Genetic diversity within populations of A. pallipes, estimated by Shannon's diversity index, ranged from 0 to 0.446 with a mean or 0.159. A UPGMA dendrogram constructed from pairwise (DST values between Populations, revealed three clusters corresponding to populations sampled in the southern, northwestern and eastern part or its range. AMOVA analysis revealed a high genetic structure of A. pallipes populations Phi (ST) = 0.814, with 73.11% of the genetic variation distributed between these clusters. It suggests it historical geographical separation of these groups into three refugial areas, probably in the Rhine, Mediterranean and Atlantic basins during recent glaciations. The close genetic relationships between English and western French populations are in accordance with a natural postglacial origin of English Populations from individuals having survived in an Atlantic refugium. However, the present results suggest that the Irish stock originated from a human translocation of individuals from an Atlantic refugium.