Multisite Performance Evaluation of an Enzyme-Linked Immunosorbent Assay for Detection of Giardia, Cryptosporidium, and Entamoeba histolytica Antigens in Human Stool

被引：17

作者：

Christy, Nathaniel C. V. ^{[1
]}

Hencke, Janice D. ^{[2
]}

Escueta-De Cadiz, Aleyla ^{[3
]}

Nazib, Forida ^{[4
]}

von Thien, Heidrun ^{[5
]}

Yagita, Kenji ^{[3
]}

Ligaba, Segni ^{[1
]}

Haque, Rashidul ^{[4
]}

Nozaki, Tomoyoshi ^{[3
,6
]}

Tannich, Egbert ^{[5
]}

Herbein, Joel F. ^{[2
]}

Petri, William A., Jr. ^{[1
]}

机构：

[1] Univ Virginia, Sch Med, Div Infect Dis & Int Hlth, Charlottesville, VA 22908 USA

[2] TechLab Inc, Blacksburg, VA USA

[3] Natl Inst Infect Dis, Dept Parasitol, Shinjuku Ku, Tokyo 1628640, Japan

[4] Int Ctr Diarrhoeal Dis Res, Dhaka 1000, Bangladesh

[5] Bernhard Nocht Inst Trop Med, Hamburg, Germany

[6] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 05期

关键词：

PRESCHOOL-CHILDREN; DIARRHEA; INFECTIONS; BANGLADESH; MALNUTRITION; ASSOCIATION; AMEBIASIS; PARASITES; LAMBLIA; BIOLOGY;

D O I：

10.1128/JCM.06483-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel fecal antigen detection assay for fresh and frozen human samples that detects but does not differentiate Giardia spp, Cryptosporidium spp, and Entamoeba histolytica, the Tri-Combo parasite screen, was compared to three established enzyme-linked immunosorbent assays (ELISAs) at three international sites. It exhibited 97.9% sensitivity and 97.0% specificity, with positive and negative predictive values of 93.4% and 99.1%, respectively. The Tri-Combo test proved a reliable means to limit the use of individual parasite ELISAs to positive samples.

引用

页码：1762 / 1763

页数：2

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