Electron Cryotomography

被引：16
作者：
Murphy, Gavin E. [1 ]
Jensen, Grant J. [1 ]
机构：
[1] CALTECH, Pasadena, CA 91125 USA
来源：
BIOTECHNIQUES | 2007年 / 43卷 / 04期
关键词：
D O I：
10.2144/000112568
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Electron cryotomography is an emerging technique that allows the structures of unique biological objects such as individual macromolecules, viruses, and ever small whole cells to be reconstructed in their near-native states in three dimensions (3-D) to an approximate 5 nm resolution. The required instrumentation, sample preparation and limitations data collection typical results, and future prospects are summarized briefly.
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页码：413 / +
页数：7
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