Molecular detection of a mixed infection of Goatpox virus, Orf virus, and Mycoplasma capricolum subsp capripneumoniae in goats

被引：26

作者：

Chu, Yuefeng ^{[1
]}

Yan, Xinmin ^{[1
]}

Gao, Pengcheng ^{[1
]}

Zhao, Ping ^{[1
]}

He, Ying ^{[1
]}

Liu, Jixing ^{[1
]}

Lu, Zhongxin ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Natl Foot & Mouth Dis Reference Lab,Key Lab Anim, Key Lab Grazing Anim Dis,Lanzhou Vet Res Inst,Min, Lanzhou, Peoples R China

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2011年 / 23卷 / 04期

关键词：

Goatpox virus; mixed infection; Mycoplasma capricolum subsp capripneumoniae; Orf virus; phylogeny; polymerase chain reaction; CONTAGIOUS CAPRINE PLEUROPNEUMONIA; DIAGNOSIS;

D O I：

10.1177/1040638711407883

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The current study investigated an outbreak of mixed infection with Goatpox virus (GTPV), Orf virus (ORFV), and Mycoplasma capricolum subsp. capripneumoniae (MCCP) that occurred on a Chinese goat farm, with a case fatality rate of 60.2%. The observed clinical signs were ecthyma and accelerated respiration with frequent coughing. Specific fragments of the p32 gene of GTPV, B2L gene of ORFV, and 16S ribosomal RNA gene of MCCP were synchronously amplified by polymerase chain reaction (PCR) from the tissues of 12 dead goats. The PCR products were cloned, sequenced, and aligned with related reference sequences in GenBank for further identification of the pathogens. The present study reports a mixed infection with GTPV, ORFV, and MCCP in goats.

引用

页码：786 / 789

页数：4

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