Specific volumes of unsaturated phosphatidylcholines in the liquid crystalline lamellar phase

被引:69
作者
Koenig, BW [1 ]
Gawrisch, K
机构
[1] Forschungszentrum Julich, IBI2, NMR,Struct Biol Inst, D-52425 Julich, Germany
[2] Univ Dusseldorf, Inst Biol Phys, D-40225 Dusseldorf, Germany
[3] NIAAA, Lab Membrane Biochem & Biophys, NIH, Bethesda, MD 20892 USA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2005年 / 1715卷 / 01期
关键词
specific volume; lipid; polyunsaturated fatty acid; DHA; neutral buoyancy method;
D O I
10.1016/j.bbamem.2005.07.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The specific volumes of seven 1,2-diacyl-sn-glycero-3-phosphocliolines with symmetric, unbranched acyl chains containing one, four, or six cis double bonds per chain, or with a saturated sn-1 chain and one, four, or six cis double bonds in the sn-2 chain were determined by the neutral buoyancy method. Experiments were conducted in the liquid crystalline lamellar phase over the temperature range from 5 to 35 degrees C. It is demonstrated that the molecular volume of phosphatidylcholines can be well approximated as the sum of a constant volume of the polar lipid head region and the temperature-dependent volumes of hydrocarbon chain -CH2, CH, and terminal CH3 groups. A linear dependence of chain segment volumes on temperature was observed. A self-consistent set of partially temperature-dependent volumes is obtained that allows prediction of phosphatidylcholine molecular volumes within very tight error margins. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:65 / 70
页数:6
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